콘텐츠로 건너뛰기
Merck
모든 사진(1)

주요 문서

모든 문서 보기

SH1131

Sigma-Aldrich

MISSION® shRNA Human Gene Family Set, Lentiviral Particles

Nuclear Hormone Receptors

로그인조직 및 계약 가격 보기
모든 사진(1)

About This Item

UNSPSC 코드:
41106609
NACRES:
NA.51

일반 설명

Lentiviral particles (representational, plate titer) are provided in 96-well plates that are barcoded for simple identification. Aliquots of 4 x 50μl are provided to prevent the need for excessive freeze/thaw, and maximize functional viral particles. Each gene family set is representationally titered (10% of clones). Fully titered sets are also available, for more information inquire at [email protected]. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.

기타 정보

Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.
For a detailed listing of other available gene family sets, visit the gene family set website.
Number of Genes: 218, Number of Clones: 1448
The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.

법적 정보

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense .
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 문서 section.

도움이 필요하시면 연락하세요. 고객 지원 부서

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

The nuclear hormone receptor gene superfamily
Ribeiro, R. C., et al.
Annals of Medicine, 46, 443-453 (1995)
Nuclear hormone receptor assays for drug discovery
Rosen, J., et al.
Drug Des. Discovery, 6, 224-230 (2003)
Coactivators in assay design for nuclear hormone receptor drug discovery
Chen, T., et al.
Drug Delivery Technology, 1, 835-842 (2003)
Miltiadis Kininis et al.
Nuclear receptor signaling, 6, e005-e005 (2008-02-28)
Recent genomic analyses of transcription factor binding, histone modification, and gene expression have provided a global view of transcriptional regulation by nuclear receptors (NRs) that complements an existing large body of literature on gene-specific studies. The picture emerging from these
J C Burns et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(17), 8033-8037 (1993-09-01)
The restricted host-cell range and low titer of retroviral vectors limit their use for stable gene transfer in eukaryotic cells. To overcome these limitations, we have produced murine leukemia virus-derived vectors in which the retroviral envelope glycoprotein has been completely
모든 관련된 서류 보기

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.