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Merck
모든 사진(2)

주요 문서

SRP0177

Sigma-Aldrich

Histone H3 full length human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

동의어(들):

HIST1H3E, Histone H3.1

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About This Item

UNSPSC 코드:
12352200
NACRES:
NA.32

생물학적 소스

human

재조합

expressed in E. coli

분석

≥80% (SDS-PAGE)

양식

aqueous solution

분자량

15.4 kDa

포장

pkg of 1 mg

저장 조건

avoid repeated freeze/thaw cycles

농도

>0.02 mg/mL

기술

activity assay: suitable

solubility

water: soluble

적합성

suitable for molecular biology

NCBI 수납 번호

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−70°C

유전자 정보

human ... HIST1H3E(8353)

일반 설명

Research area: Cell signaling. The gene HIST1H3E (histone 3, also referred to as H3.1) is mapped to human chromosome 6p22. It is a variant of histone 3 and is also called the replicative histone. HIST1H3E is mainly expressed in the S-phase. In the process of cellular differentiation, HIST1H3E mRNA levels go down as cell division slows down.Human HIST1H3E, GenBank Accession No. NM_003532, amino acids 2-137 (end) with N-terminal His-tag, MW = 15.4kDa, expressed in an Escherichia coli expression system.

애플리케이션

Histone H3 full-length human has been used as a substrate in in vitro kinase assay for Aurora-A (AURKA) kinase and Tousled-Like Kinase 2(TLK2). Histone H3 full-length human has been used in the generation of antibodies in chickens. It has also been used in in vitro methylation assay.

생화학적/생리학적 작용

Histones are important for proper organization and modification of chromatin. HIST1H3E (histone 3, also referred to as H3.1) is mainly associated with DNA synthesis during DNA replication and might also be involved in DNA repair. It is added to the chromatin at the time of DNA replication-associated chromatin assembly. During HSV-1 (herpes simplex virus 1) infection, differential movement of HIST1H3E is associated with the assembly of viral chromatin.

물리적 형태

Formulated in 10 mM PBS, pH 7.4, 130 mM NaCl, 10% glycerol and 1 mM DTT.

제조 메모

Thaw on ice. Upon first thaw, briefly spin tube containing protein to recover full content of the tube. Aliquot protein into single use aliquots. Store remaining undiluted protein in aliquots at -70°C. Note: Protein is very sensitive to freeze/thaw cycles.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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Jona Merx et al.
Scientific reports, 12(1), 19251-19251 (2022-11-11)
Posttranslational modifications (PTMs) on histone tails regulate eukaryotic gene expression by impacting the chromatin structure and by modulating interactions with other cellular proteins. One such PTM has been identified as serine and threonine glycosylation, the introduction of the ß-N-acetylglucosamine (GlcNAc)
The human histone H3 complement anno 2011.
Ederveen TH
Biochimica et Biophysica Acta, 1809, 577-586 (2011)
Histone H3.1 and H3.3 complexes mediate nucleosome assembly pathways dependent or independent of DNA synthesis.
Tagami H
Cell, 116, 51-61 (2004)
Method of Immunoglobulin Y (IgY) Extraction for Minimally Toxic In Vivo Applications
Nevado Jr, et al.
Philippine journal of Science, 153(1) (2024)
Kami Ahmad et al.
Molecular cell, 9(6), 1191-1200 (2002-06-28)
Two very similar H3 histones-differing at only four amino acid positions-are produced in Drosophila cells. Here we describe a mechanism of chromatin regulation whereby the variant H3.3 is deposited at particular loci, including active rDNA arrays. While the major H3

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