SRP0291

Cathepsin L Active human

recombinant, expressed in FreeStyle^™ 293-F cells, ≥90% (SDS-PAGE)

• 동의어(들): CATL, Major excreted protein (MEP)
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: human
• 재조합: expressed in FreeStyle^™ 293-F cells
• 분석: ≥90% (SDS-PAGE)
• 양식: aqueous solution
• 특이 활성도: ≥3900 pmol/min-μg
• 분자량: 36 kDa
• 기술: inhibition assay: suitable
• 적합성: suitable for molecular biology
• NCBI 수납 번호: NM_001912
• 응용 분야: life science and biopharma
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... CTSL(1514)

설명

일반 설명

Research area: Cell Signaling

Cathepsin L is a papain-like cysteine protease and belongs to the Clan A, Family C1. It is composed of L domain of α-helix and an R domain of β-sheet in the spatial structure.

Human cathepsin L (GenBank Accession No. NM_001912), amino acids 18-333, with C-terminal HIS tag, MW = 36 kDa, expressed in FreeStyle 293-F cells.

애플리케이션

Active human cathepsin L is useful for the activation of DPP1 (Cathepsin C). Active human cathepsin L has been used:

• to determine that N-acyl and N-sulfonyloxazolidine-2,4-diones are pseudo-irreversible inhibitors of serine proteases
• to investigate the role of cathepsin B and L activity in the serum during the human aging process.
• in inhibitory activity assay
• to study the role of CTSL in COVID-19 infection

생화학적/생리학적 작용

Cathepsin L is a cysteine protease, involved primarily in protein breakdown in the lysosome. Cathepsin L degrades nuclear transcription factors and may affect cell cycle regulation. Cathepsin L is also involved in the immune system, specifically in degrading the invariant chain during major histocompatibility complex (MHC) class II processing, a crucial step in antigen presentation. Its expression in the thymus has been demonstrated to be vital for the development of natural killer cells. Additionally, Cathepsin L contributes to recycling processes during axon outgrowth and synapse formation in the developing postnatal central nervous system. Proteases are associated with the development and progression of cancer. Therefore, cathepsin L may be a potential therapeutic target in cancer treatment.

단위 정의

One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C

물리적 형태

Formulated in 50 mM MES, 400 mM NaCl, pH 5.0, and 10% glycerol.

제조 메모

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

법적 정보

FreeStyle is a trademark of Invitrogen Corp.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable