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Merck
모든 사진(1)

주요 문서

SRP0293

Sigma-Aldrich

Cathepsin V Active human

recombinant, expressed in FreeStyle 293-F cells, ≥90% (SDS-PAGE)

동의어(들):

Cathepsin L2, Cathepsin U

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About This Item

UNSPSC 코드:
12352204
NACRES:
NA.54

재조합

expressed in FreeStyle 293-F cells

분석

≥90% (SDS-PAGE)

양식

aqueous solution

특이 활성도

≥436 pmol/min-μg

분자량

37 kDa

NCBI 수납 번호

배송 상태

dry ice

저장 온도

−70°C

유전자 정보

human ... CTSL2(1515)

일반 설명

Human cathepsin V (GenBank Accession No. NM_001333.2), CD33 signal peptide(amino acids 1-16) + cathepsin V (amino acids 18-334), with C-terminal HIS tag, MW = 37 kDa, expressed in FreeStyle 293-F cells.

애플리케이션

Active human cathepsin V is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human cathepsin V has been used in a study to assess the cysteine protease attribute of the eukaryotic ribosomal protein S4. Active human cathepsin V has also been used in a study to determine that the simultaneous treatment of human bronchial epithelial cells with serine and cysteine protease inhibitors prevents severe acute respiratory syndrome coronavirus entry.

단위 정의

One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C

물리적 형태

Formulated in 40 mM MES, pH 5.0, 120 mM NaCL and 20% glycerol.

제조 메모

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

법적 정보

FreeStyle is a trademark of Invitrogen Corp.

픽토그램

Health hazardExclamation mark

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

Miyuki Kawase et al.
Journal of virology, 86(12), 6537-6545 (2012-04-13)
The type II transmembrane protease TMPRSS2 activates the spike (S) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) on the cell surface following receptor binding during viral entry into cells. In the absence of TMPRSS2, SARS-CoV achieves cell entry via
Babu Sudhamalla et al.
Biochimica et biophysica acta, 1820(10), 1535-1542 (2012-05-15)
Ribosomal proteins often carry out extraribosomal functions. The protein S4 from the smaller subunit of Escherichia coli, for instance, regulates self synthesis and acts as a transcription factor. In humans, S4 might be involved in Turner syndrome. Recent studies also
Uta Schurigt et al.
Inflammation research : official journal of the European Histamine Research Society ... [et al.], 61(9), 1021-1029 (2012-06-08)
Cathepsin L (CL) is potentially involved in joint destruction and in antigen presentation in rheumatoid arthritis. In order to define the roles of this protease in arthritis development we analysed the antigen-induced arthritis (AIA) in CL-deficient (CL(-/-)) mice. Antigen-induced arthritis
Ryan P McGlinchey et al.
Biochemical and biophysical research communications, 529(4), 1106-1111 (2020-08-21)
The intracellular accumulation of α-synuclein (α-syn) amyloid fibrils is a hallmark of Parkinson's disease. Because lysosomes are responsible for degrading aggregated species, enhancing lysosomal function could alleviate the overburden of α-syn. Previously, we showed that cysteine cathepsins (Cts) is the

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