T1378

Sigma 7-9^®

≥99% (titration), crystalline, Sigma 7-9^®

• 동의어(들): Trizma^® base, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
• Linear Formula: NH₂C(CH₂OH)₃
• CAS Number: 77-86-1
• Molecular Weight: 121.14
• Beilstein: 741883
• EC Number: 201-064-4
• MDL number: MFCD00004679
• UNSPSC 코드: 12352104
• PubChem Substance ID: 24899961
• NACRES: NA.25

속성

• 제품명: Sigma 7-9^®, ≥99% (titration), crystalline
• 설명: aminopeptidase substrate
• Quality Level: 200
• 분석: ≥99% (titration)
• 양식: crystalline
• 저장 조건: dry at room temperature
• 기술: ELISA: suitable; flow cytometry: suitable; immunohistochemistry: suitable
• 불순물: ≤0.5% water (Karl Fischer)
• 색상: white to off-white
• pH: 10.5-12
• 유용한 pH 범위: 7-9
• pKa(25 °C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.)
• solubility: water: 0.667 g/mL, clear, colorless to faintly yellow
• 흡수: ≤0.4 at 290 at 40%
• 응용 분야: clinical research; diagnostic assay manufacturing; life science and biopharma; sample preparation
• SMILES string: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChI key: LENZDBCJOHFCAS-UHFFFAOYSA-N

설명

일반 설명

Sigma 7-9^® also known as Trizma base or tris(hydroxymethyl)aminomethane, is a versatile biological buffer used in various applications, such as protein freezing, electrophoresis, and the preparation of buffer solutions such as TAE and TBE. With a pKa of 8.06, Tris is particularly valuable in labs working within the typical physiological pH range of living organisms (pH 7.0-9.0). It is also commonly employed in combination with other buffering agents to precisely adjust solution pH.

Additionally, Tris aids in enhancing the permeability of cell membranes. This compound is indispensable in laboratories where it is utilized to prepare buffers compatible with biological fluids, serve as a standard pH solution, and facilitate procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells.

애플리케이션

Sigma 7-9^® has been used:

• as a buffer for sample preparation in a study involving Enzyme-linked immunosorbent assay (ELISA)
• as a buffer for sample preparation in research involving immunohistochemistry and immunofluorescence

특징 및 장점

• Suitable for Biological and Biochemical Research
• Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)

기타 정보

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

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법적 정보

Sigma 7-9 is a registered trademark of Merck KGaA, Darmstadt, Germany

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Gloves