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Merck
모든 사진(1)

주요 문서

T2194

Sigma-Aldrich

Trizma® hydrochloride solution

pH 7.4, BioPerformance Certified, 1 M, suitable for cell culture

동의어(들):

Tris hydrochloride solution

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모든 사진(1)

About This Item

UNSPSC 코드:
12161700
NACRES:
NA.25

Grade

BioPerformance Certified
for molecular biology

Quality Level

200

양식

solution

농도

1 M

기술

cell culture | mammalian: suitable

불순물

DNase, RNase, Protease, none detected
 bioburden, tested
 endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH

7.4

유용한 pH 범위

7.0-9.0

흡수

≤0.05 at 290 at 40%

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일반 설명

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

애플리케이션

Trizma®hydrochloride solution has been used:
  • in the preparation of cell-lysis buffer
  • as a component of wash buffers and RNase mix 1, used in the preparation of 5′-complete cDNAs for paired-end sequencing
  • in dissolving cadmium or S2- loaded microreactors for CdS synthesis

법적 정보

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
0000398891
0000398891
0000398892
0000398892
SLCR1844

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

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Pacheco M, et al.
Chemical Science, 9(42), 8056-8064 (2018)
RAMPAGE: Promoter Activity Profiling by Paired-End Sequencing of 5?-Complete cDNAs
Batut P and Gingeras TR
Current Protocols in Molecular Biology, 104(1), 25B-211 (2013)
Determination of Rate of [3H-methyl]-choline Incorporation into Cellular Lipids and Non-lipid Metabolites
Smith T and Phyu S
Bio-protocol, 6(22) (2016)
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STAR protocols, 2(3), 100694-100694 (2021-08-13)
Single-nucleus RNA sequencing allows the profiling of gene expression in isolated nuclei. Here, we describe a step-by-step protocol optimized for adult mouse skeletal muscles. This protocol provides two main advantages compared to the widely used single-cell protocol. First, it allows
Kenneth Shatzkes et al.
Scientific reports, 4, 4659-4659 (2014-04-12)
Sample nucleic acid purification can often be rate-limiting for conventional quantitative PCR (qPCR) workflows. We recently developed high-throughput virus microneutralization assays using an endpoint assessment approach based on reverse transcription qPCR (RT-qPCR). The need for cumbersome RNA purification is circumvented
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