콘텐츠로 건너뛰기
Merck
모든 사진(2)

주요 문서

T9157

Sigma-Aldrich

Tryptose Phosphate Broth

suitable for insect cell culture

동의어(들):

Tryptose Broth

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
41106507
NACRES:
NA.73

생물학적 소스

bovine milk

Quality Level

형태

solid

기술

cell culture | insect: suitable

배송 상태

ambient

저장 온도

room temp

일반 설명

Tryptose Phosphate Broth (TPB) comprises pyrimidine based components and is crucial for pyrimidine synthesis and electron transport chain in cells. In chick embryo fibroblast, it alleviates chloramphenicol inhibition.

애플리케이션

In addition to its use for the growth of fastidious micro-organisms, Tryptose Phospate Broth (TPB) has been studied as supplement for the preparation of media that supports vaccine production in BHK-21 cells and the growth of SF21 insect cells in high-density perfusion culture stirred-tank bioreactors.
Tryptose Phosphate Broth has been used as a supplement:
  • in Eagle′s minimum essential medium alpha for growing Vero cells
  • for Vero-CCL81 cells in porcine epidemic diarrhea coronavirus propagation
  • in Dulbecco modified Eagle medium in LR7cells for mouse hepatitis virus propagation
  • in Glasgow minimum essential medium (GMEM) for Madin-Darby canine kidney (MDCK) cells.

성분

Tryptose Phosphate Broth (TPB) is composed of four components: Tryptose (20g/L); Dextrose (2g/L); NaCl (5g/L) and Disodium Phosphate (2.5g/L) typically adjusted to pH 7.3. The tryptose component is a peptone (mixture of amino acids and short peptides) derived by the mixed enzymatic hydrolysis (pancreatic enzymes) of the milk protein casein. This hydrolysate provides a source of amino acid based nutrients and survival factors that support the growth of fastidious micro-organisms such as Brucella, Streptococcus, and Neisseria; as well as eukaryotic cells such as insect and animal cells. Dextrose provides a fermentable carbohydrate that can be used by fastidious mico-organisms. Sodium chloride maintains the osmotic and ionic equilibrium and disodium phosphate provides the basic buffering capacity.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Identification and characterization of a proteolytically primed form of the murine coronavirus spike proteins after fusion with the target cell
Wicht O, et al.
Journal of Virology, 88(9), 4943-4952 (2014)
Phytochemical analysis and in-vitro screening of selected Indian medicinal plants for antiviral activity against highly pathogenic avian influenza virus
Sood R, et al.
Spatula DD, 3(3), 81-88 (2013)
Tryptose phosphate broth improves Rickettsia felis replication in mammalian cells
Saisongkorh W, et al.
FEMS Immunology and Medical Microbiology, 64(1), 111-114 (2012)
Aminopeptidase N is not required for porcine epidemic diarrhea virus cell entry
Li W, et al.
Virus Research, 235, 6-13 (2017)
Azali Azlan et al.
PLoS neglected tropical diseases, 15(1), e0008351-e0008351 (2021-01-23)
The Asian tiger mosquito, Aedes albopictus (Ae. albopictus), is an important vector that transmits arboviruses such as dengue (DENV), Zika (ZIKV) and Chikungunya virus (CHIKV). Long noncoding RNAs (lncRNAs) are known to regulate various biological processes. Knowledge on Ae. albopictus

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.