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Merck
모든 사진(1)

주요 문서

T8159

Sigma-Aldrich

Tryptose Phosphate Broth solution

sterile-filtered, suitable for cell culture

동의어(들):

TPB solution

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About This Item

UNSPSC 코드:
12352205
NACRES:
NA.75

무균

sterile-filtered

Quality Level

형태

solution

농도

29.5 g/L in deionized water

기술

cell culture | mammalian: suitable

배송 상태

ambient

저장 온도

room temp

애플리케이션

In addition to its use for the growth of fastidious micro-organisms, Tryptose Phospate Broth (TPB) has been studied as supplement for the preparation of media that supports vaccine production in BHK-21 cells and the growth of SF21 insect cells in high-density perfusion culture stirred-tank bioreactors.

성분

Tryptose Phosphate Broth (TPB) is composed of four components: Tryptose (20g/L); Dextrose (2g/L); NaCl (5g/L) and Disodium Phosphate (2.5g/L) typically adjusted to pH 7.3. The tryptose component is a peptone (mixture of amino acids and short peptides) derived by the mixed enzymatic hydrolysis (pancreatic enzymes) of the milk protein casein. This hydrolysate provides a source of amino acid based nutrients and survival factors that support the growth of fastidious micro-organisms such as Brucella, Streptococcus, and Neisseria; as well as eukaryotic cells such as insect and animal cells. Dextrose provides a fermentable carbohydrate that can be used by fastidious mico-organisms. Sodium chloride maintains the osmotic and ionic equilibrium and disodium phosphate provides the basic buffering capacity.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

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