T9378

Trypsin inhibitor

crude powder

• 동의어(들): Trypsin Inhibitor from lima beans
• CAS Number: 9035-81-8
• EC Number: 232-906-9
• MDL number: MFCD00082102
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• product name: Trypsin inhibitor from Phaseolus limensis (lima bean), Type II-L, crude powder
• 생물학적 소스: Phaseolus limensis (lima bean)
• Quality Level: 200
• 유형: Type II-L
• 형태: crude powder
• 분자량: 9 kDa
• solubility: 0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL
• 저장 온도: 2-8°C

설명

애플리케이션

Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:

• in the inhibition of trypsin in human plasma
• in the termination of proteolytic digestion in rabbit skeletal muscles
• in the inhibition of salivary glands enzyme extract from Lygus lineolaris
• in the inhibition of proteolytic activity in Agave tequilana enzyme extract

Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type enzymes.

생화학적/생리학적 작용

Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors. It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.

물리적 특성

Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.

단위 정의

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

제조 메모

Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).

분석 메모

One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

기타 정보

View more information on Trypsin Inhibitor.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H317,H334
• 예방조치 성명서: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)