추천 제품
생물학적 소스
soybean
Quality Level
양식
lyophilized
분자량
Mr 20,100
포장
pkg of 50 mg
제조업체/상표
Roche
농도
1 mg/mL
기술
cell culture | mammalian: suitable
pH
3.8-4.5
solubility
water: soluble
저장 온도
2-8°C
일반 설명
Soybean trypsin inhibitor (STI) comprises 181 amino acids. It is a monomeric protein corresponding to a molecular weight of 20 kDa and has two disulfide bridges. It belongs to serpins − serine protease inhibitors family.
특이성
Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. It inhibits trypsin, factor Xa, plasmin, and plasma kallikrein activity in serum-free cell culture media. It does not inhibit metallo-, cysteine, aspartic proteases, or tissue kallikrein (serine proteases).
애플리케이션
Trypsin Inhibitor from soybean is used to terminate tissue disaggregation and for subcultivation procedures.
Trypsin Inhibitor has been used:
- in the buffer for hepatocyte isolation
- to inhibit trypsin in Corneal epithelial cells
- to inhibit papain activity while treating hippocampal neural stem cells (NSCs)
생화학적/생리학적 작용
Soybean trypsin inhibitor (STI) inhibits myc proto-oncogene protein and is anticarcinogenic. It favors anti-aggregation of platelets and may be a potential protease inhibiting drug. STI inhibits renin and angiotensin.
Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. The product is sensitive to heat, alkaline pH, and protein-precipitating compounds.
규격
Inhibiting activity: approximately 70 trypsin inhibitor units/mg lyophilizate at +25°C with benzoyl-L-arginine ethylester as a substrate (approximately 190 trypsin inhibitor units/mg at +25°C with Chromozym TRY as substrate)
Isoelectric point: 4.5
Isoelectric point: 4.5
단위 정의
1 U = 270 S & T-units or 90 USP-units
제조 메모
Working concentration: 10 to 100 μg/ml
Working solution: Recommended solvent is distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
The enzyme is stable for 6 months, frozen in aliquots.
Working solution: Recommended solvent is distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
The enzyme is stable for 6 months, frozen in aliquots.
재구성
Sensitive to heat, alkaline pH, and protein-precipitating compounds.
저장 및 안정성
Store at 2 to 8 °C. (Store dry!)
기타 정보
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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