796-0657

HPLC Analysis of Proteins on Discovery^® BIO PolyMA-WAX (Analyte Set #1)

application for HPLC

속성

• 설명: Separated on Discovery^® BIO PolyMA-WAX
• 기술: HPLC: suitable
• 검사 파라미터: column: Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U); mobile phase: [A] 10 mM Tris, pH 8.0 with HCl; [B] 10 mM Tris, 0.5 M NaCl, pH 8.0 with HCl; 5 to 100% B in 15 min. (linear); flow rate: 0.5 mL/min; detector: UV, 280 nm; injection: 10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A
• 적합성: application for HPLC
• 응용 분야: life science and biopharma

설명

분석 메모

The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the separation of proteins that vary from 18 to 80 kDA.

법적 정보

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

분석물

Trypsin Inhibitor