G005697

HPLC Analysis of Fat Soluble Vitamins by Normal Phase Chromatography on Ascentis^® Si (25 cm Column)

application for HPLC

속성

• 기술: HPLC: suitable
• 검사 파라미터: column: Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U); mobile phase: [A] hexane; [B] ethyl acetate; gradient: 10 to 30% B in 10 min; held at 30% B for 2 min; flow rate: 1.0 mL/min; column temp.: 30 °C; detector: UV, 290 nm; injection: 10 μL
• 적합성: application for HPLC
• 응용 분야: vitamins, nutraceuticals, and natural products

설명

분석 메모

Ascentis silica is used in the analysis of fat-soluble vitamins using a normal phase gradient. These include vitamin K 3 (menadione), vitamin E (alpha and gamma tocopherol), and vitamin D3 (cholecalciferol).

법적 정보

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

분석물

(±)-α-Tocopherol, analytical standard
Menadione (K3), analytical standard
(+)-γ-Tocopherol, analytical standard
Cholecalciferol (D3), analytical standard