G005876

LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis^® Express F5 (Monitored Ions)

application for HPLC

속성

• 기술: HPLC: suitable
• 검사 파라미터: column: Ascentis Express F5, 15 cm x 2.1 mm I.D., 2.7 μm particles (53571-U); mobile phase: [A] 5 mM ammonium formate water; [B] methanol; (25:75, A:B); flow rate: 0.4 mL/min; pressure: 5178 psi (357 bar); column temp.: 40 °C; detector: ESI(+), 100-1000 m/z; injection: 2 μL, each compound 300 ng/mL in 40:60 (water:methanol)
• 적합성: application for HPLC
• 응용 분야: clinical; vitamins, nutraceuticals, and natural products

설명

분석 메모

The metabolic role and health implications of the various vitamin D isoforms are of current clinical interest. Therefore, it is important to have an analytical method that will resolve all of the known isoforms with necessary sensitivity and specificity.This application shows that a combination of Ascentis Express F5 with MS detection provided rapid resolution of seven such compounds. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

법적 정보

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

분석물

1α,25-Dihydroxyvitamin D3 solution, 5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant^®
1α,25-Dihydroxyvitamin D2 solution, 5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant^®
25-Hydroxyvitamin D3 solution, 100 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant^®
3-epi-25-Hydroxyvitamin D₃, 98% (CP)
3-epi-25-Hydroxyvitamin D₂ solution, 100 μg/mL in ethanol, 98% (CP)
1α-Hydroxyvitamin D₃, ≥97.0% (HPLC)