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D7008

Sigma-Aldrich

Dihydroethidium

≥95%

Synonym(s):
2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine, Hydroethidine
Empirical Formula (Hill Notation):
C21H21N3
CAS Number:
Molecular Weight:
315.41
Beilstein:
5107482
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

Assay

≥95%

form

powder

solubility

chloroform: 20 mg/mL

fluorescence

λex 370 nm; λem 420 nm (for cytoplasm of living cells)
λex 535 nm; λem 610 nm (for chromatin of living cells)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CCN1C(c2ccccc2)c3cc(N)ccc3-c4ccc(N)cc14

InChI

1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3

InChI key

XYJODUBPWNZLML-UHFFFAOYSA-N

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This Item
37291D4784L0259
Dihydroethidium ≥95%

Sigma-Aldrich

D7008

Dihydroethidium

Dihydroethidium BioReagent, suitable for fluorescence, ≥95% (HPCE)

Sigma-Aldrich

37291

Dihydroethidium

form

powder

form

solid

form

powder

form

powder

solubility

chloroform: 20 mg/mL

solubility

acetonitrile: soluble, methanol: soluble

solubility

H2O: 50 mg/mL

solubility

H2O: 1 mg/mL

fluorescence

λex 370 nm; λem 420 nm (for cytoplasm of living cells), λex 535 nm; λem 610 nm (for chromatin of living cells)

fluorescence

λex 358 nm; λem 461 nm, λex 392 nm; λem 410 nm in methanol

fluorescence

-

fluorescence

λex 428 nm; λem 540 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Application

Redox indicator. Blue fluorescence until oxidized to ethidium.
Dihydroethidium has been used as a superoxide probe in bovine pulmonary artery endothelial cell (BPAEC). It has been used to check the in situ generation of ROS (reactive oxygen species).

Packaging

Packaged under Argon.

Linkage

B-ring reduced analog of ethidium

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Articles

Mitochondrial Stress and ROS

Oxidative stress is mediated, in part, by reactive oxygen species produced by multiple cellular processes and controlled by cellular antioxidant mechanisms such as enzymatic scavengers or antioxidant modulators. Free radicals, such as reactive oxygen species, cause cellular damage via cellular.

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