MilliporeSigma
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917575

Sigma-Aldrich

PhotoCol-IRG, methacrylated collagen bioink kit, with Irgacure

Synonym(s):
3D Bioprinting, Bioink, Collagen

description

Methacrylated collagen:
Degree of methacrylation ≥ 20%

Product components :
Methacrylated collagen (100 mg)
20 mM acetic acid (50 mL)
Neutralization solution (10 mL)
Irgacure photoinitiator ( 100 mg)

Quality Level

sterility

sterile; sterile-filtered

impurities

≤10 EU/mL Endotoxin

storage temp.

2-8°C

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917834CC320-1CC320-2
description

Methacrylated collagen:
Degree of methacrylation ≥ 20%

description

Methacrylated collagen:
Degree of methacrylation ≥ 20%, Product components :
Methacrylated collagen (100 mg)
20 mM acetic acid (50 mL)
Neutralization solution (10 mL)
, Ruthenium (100 mg)
Sodium persulfate photoinitiator (500 mg)

description

-

description

-

sterility

sterile; sterile-filtered

sterility

sterile; sterile-filtered

sterility

-

sterility

-

impurities

≤10 EU/mL Endotoxin

impurities

≤10 EU/mL Endotoxin

impurities

-

impurities

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

-

Quality Level

100

Quality Level

100

Quality Level

-

Quality Level

-

Application

PhotoCol-IRG bioink kit consists of purified methacrylated Type I bovine collagen as the core component with other support reagents. The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen where the collagen has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the a-amines groups on the N-termini. Over 20% of the total lysine residues of the collagen molecule have been methacrylated. A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml. The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture. The photoinitiator consists of Irgacure 2959 to be formulated in methanol (methanol not included), which allows UV crosslinking of the printed structure at 365 nm. PhotoCol-IRG provides native-like 3D collagen gels, and the final gel stiffness can be customized by changing collagen concentrations and crosslinking.

Legal Information

PhotoCol is a trademark of Advanced BioMatrix, Inc.

Pictograms

Environment

Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


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