925055

TissueFab^® GelAlg − LAP Bioink

low endotoxin, 0.2 μm filtered, suitable for 3D bioprinting applications

• Synonym(s): GelMA-alginate bioink

Properties

• Quality Level: 100
• sterility: 0.2 μm filtered
• form: viscous liquid (gel)
• impurities: <5 cfu/mL Bioburden; <50 EU/mL Endotoxin
• color: pale yellow to colorless
• pH: 6.5-7.5
• viscosity: 10-40 cP
• application(s): 3D bioprinting
• storage temp.: 2-8°C

Description

General description

Gelatin methacryloyl (GelMA) is a polymerizable hydrogel material derived from natural extracellular matrix (ECM) components. Due to its low cost, abundance, and retention of natural cell-binding motifs, gelatin has become a highly sought material for tissue engineering applications.

Alginate is a naturally occurring polymer widely applied for bioprinting applications as its printability can be easily modified by altering the polymer density and crosslinking with the addition of calcium chloride (CaCl₂). Alginate is often combined with gelatin to facilitate cell adhesion and differentiation.

Temporal and spatial control of the crosslinking reaction can be obtained by adjusting the degree of functionalization and polymerization conditions, allowing for the fabrication of hydrogels with unique patterns, 3D structures, and morphologies.

Application

Gelatin methacrylate based bioinks have been used in the following bioprinting applications:

• osteogenic [1],
• chondrogenic [2] [3],
• hepatic [4] [5] [6],
• adipogenic [7],
• vasculogenic [8],
• epithelial [6],
• endothelial [9] [10],
• cardiac valve [11],
• skin [12],
• tumors [10]

Features and Benefits

• Ready-to-use formulation optimized for high printing fidelity and cell viability, eliminating the lengthy bioink formulation development process
• Step-by-step protocols developed and tested by MilliporeSigma 3D Bioprinting Scientists, no prior 3D bioprinting experience needed
• Suitable for different extrusion-based 3D bioprinter model
• Methacrylamide functional group can also be used to control the hydrogel physical parameters such as pore size, degradation rate, and swell ratio.

Legal Information

TISSUEFAB is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3