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Key Documents

05-466

Sigma-Aldrich

Anti-GRK 4-6 Antibody, clone A16/17

clone A16/17, Upstate®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

A16/17, monoclonal

species reactivity

mouse, human, rat

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

mouse ... Grk4(14772)

Specificity

GRK 4, GRK 5 and GRK 6

Immunogen

GST fusion protein corresponding to residues 463-590 of bovine GRK 5

Application

Anti-GRK 4-6 Antibody, clone A16/17 detects level of GRK 4-6 & has been published & validated for use in IP & WB.
Research Category
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

Quality

routinely evaluated by immunoblot on murine 3T3/A31 RIPA lysate or human K562 cells

Target description

65-70kDa

Physical form

0.1M Tris-glycine, pH 7.4, 0.15 NaCl with 0.05% sodium azide
Ammonium Sulfate Precipitation
Format: Purified

Storage and Stability

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Sudipto Das et al.
Pflugers Archiv : European journal of physiology, 464(1), 101-109 (2012-04-25)
Mitochondrial structure and function are central to cell physiology and are mutually interdependent. Mitochondria represent a primary target of the alcohol-induced tissue injury, particularly in the liver, where the metabolic effects of ethanol are predominant. However, the effect of ethanol
Rachel M DeRita et al.
Journal of cellular biochemistry, 118(1), 66-73 (2016-05-28)
It is well known that Src tyrosine kinase, insulin-like growth factor 1 receptor (IGF-IR), and focal adhesion kinase (FAK) play important roles in prostate cancer (PrCa) development and progression. Src, which signals through FAK in response to integrin activation, has
E R Bychkov et al.
Neurobiology of aging, 29(3), 379-396 (2006-11-28)
Arrestins and G proteins-coupled receptor kinases (GRKs) regulate signaling and trafficking of G protein-coupled receptors. We investigated changes in the expression of arrestins and GRKs in the striatum of patients with Parkinson's disease without (PD) or with dementia (PDD) at
Y Nagayama et al.
The Journal of biological chemistry, 271(17), 10143-10148 (1996-04-26)
Homologous desensitization of G protein-coupled receptors involves agonist-dependent phosphorylation of receptors by G protein-coupled receptor kinases (GRKs). To identify GRK(s) that play a role in homologous desensitization of the thyrotropin (TSH) receptor, thyroid cDNA was amplified by polymerase chain reaction
Cierra A Birch et al.
Frontiers in molecular biosciences, 10, 1211597-1211597 (2023-09-11)
Introduction: Dysfunction of the endothelium impairs its' protective role and promotes inflammation and progression of vascular diseases. Activated Protein C (APC) elicits endothelial cytoprotective responses including barrier stabilization, anti-inflammatory and anti-apoptotic responses through the activation of the G protein-coupled receptor

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