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F4049

Sigma-Aldrich

Monoclonal ANTI-FLAG® M2-FITC antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous solution

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Synonym(s):
Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Anti-ddddk, Anti-dykddddk
NACRES:
NA.32

biological source

mouse

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous solution

species reactivity

all

concentration

~1 mg/mL

technique(s)

direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

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This Item
A9594F3165F9291
conjugate

FITC conjugate

conjugate

CY3 conjugate

conjugate

unconjugated

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin (Purified IgG1 subclass)

antibody form

purified immunoglobulin

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

form

buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

form

buffered aqueous glycerol solution

species reactivity

all

species reactivity

all

species reactivity

all

species reactivity

all

General description

Anti Flag M2-FITC antibody is used for the detection of Flag fusion proteins.This monoclonal antibody is produced in mouse and covalently conjugated to flourescein isothiocyanate (FITC). The antibody recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus.Binding is not Calcium dependent. F4049 is an Affinity purified antibody, increasing sensitivity in most applications.

Application

Antibody is suitable for immunofluorescence, immunocytochemistry, immunohistochemistry, immunoblotting, flow cytometry and electron microscopy. Ideal for use when detection of Flag fusion proteins is required in a murine host, where secondary antibodies could cause cross reactivity.

Learn more product details in ourFLAG® application portal.

Physical form

Solution in 10 mM phosphate buffered saline, plus 1% BSA and 0.1% sodium azide, pH 7.4

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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