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A8592

Sigma-Aldrich

Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

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Synonym(s):
Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Anti-ddddk, Anti-dykddddk
NACRES:
NA.32

biological source

mouse

Quality Level

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous glycerol solution

species reactivity

all

concentration

~1 mg/mL

technique(s)

indirect ELISA: 1:20,000

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... GPX1(2876)

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This Item
A9469F1804SAB4200119
species reactivity

all

species reactivity

all

species reactivity

all

species reactivity

all

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

clone

6F7, monoclonal

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

unconjugated

conjugate

peroxidase conjugate

technique(s)

indirect ELISA: 1:20,000

technique(s)

indirect ELISA: 1:20,000

technique(s)

-

technique(s)

western blot: 1:1,000-1:2,000 using extracts of transfected cells expressing C-terminal FLAG-tagged fusion protein

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General description

The Monoclonal ANTI-FLAG M2-Peroxidase is a mouse IgG antibody covalently conjugated to horseradish peroxidase ( HRP). The antibody binds to FLAG fusion proteins and recognizes the FLAG epitope at N-terminal, Met-N-terminal, C-terminal, and internal FLAG peptides.

Application

For simple, one-step detection by immunocytochemistry, ELISA, or Western blotting. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies may cause cross-reactivity.
Suggested dilution for immunocytochemistry and western blotting 1:100 to 1:1000
Suggested dilution for ELISA 1:20,000

Learn more product details in our FLAG® application portal.

Physical form

Solution in phosphate buffered saline containing 50% glycerol plus preservative and stabilizer

Preparation Note

Dilute ANTI-FLAG M2-Peroxidase solution in Tris Buffered Saline (TBS): 0.05 M Tris, pH 7.4, with 0.15 M NaCl.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1C - Skin Sens. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Structural modifications of proteins are essential to living cells. When aberrantly regulated they are often the basis of disease. Glycans are responsible for much of the structural variation in biologic systems, and their representation on cell surfaces is commonly called the “glycome.”

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