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05-665-AF647

Sigma-Aldrich

Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor 647

clone 8E7, from mouse, ALEXA FLUOR 647

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Synonym(s):
Catenin beta-1, Beta-catenin, Active-β-Catenin
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

ALEXA FLUOR 647

antibody form

purified antibody

antibody product type

primary antibodies

clone

8E7, monoclonal

species reactivity

human, mouse

species reactivity (predicted by homology)

zebrafish (based on 100% sequence homology), rat (based on 100% sequence homology)

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CTNNB1(1499)
mouse ... Ctnnb1(12387)
rat ... Ctnnb1(84353)
zebrafish ... Ctnnb1(30265)

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vibrant-m

06-734

Anti-β-Catenin Antibody

Gene Information

human ... CTNNB1(1499)
mouse ... Ctnnb1(12387)
rat ... Ctnnb1(84353)
zebrafish ... Ctnnb1(30265)

Gene Information

human ... CTNNB1(1499)
mouse ... Ctnnb1(12387)
rat ... Ctnnb1(84353)

Gene Information

human ... CTNNB1(1499)
mouse ... Ctnnb1(12387)
rat ... Ctnnb1(84353)

Gene Information

human ... CTNNB1(1499)
mouse ... Ctnnb1(12387)

conjugate

ALEXA FLUOR 647

conjugate

-

conjugate

-

conjugate

-

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

species reactivity

human, mouse

species reactivity

human, rat, rabbit, bovine, mouse

species reactivity

rat, mouse, human

species reactivity

human

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

rat

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General description

Catenin beta-1 (UniProt P35222; also known as beta-Catenin) is encoded by the CTNNB1 (also known as CTNNB, MRD19) gene (ORF OK/SW-cl.35, PRO2286; Gene ID 1499) in human. beta-Catenin is a multifunctional protein involved in the regulation of cell–cell adhesion and in the control of the Wnt signaling pathway. beta-Catenin contains in its central region 12 armadillo repeats that mediate its interaction with a variety of proteins including axin, adenomatous polyposis coli (APC), and T cell factor (TCF) family transcription factors. The C-terminal domain (CTD) of beta-catenin is implicated in transcriptional functions, while the N-terminal domain (NTD) contains several posttranslational modification (ubiquitination, acetylation, and phosphorylation) sites, including the Ser33/Ser37/Thr41 residues, important for beta-catenin stability regulation. In the absence of Wnt pathway activation, beta-catenin is retained in the cytoplasm in a “destruction complex” with Axin, APC, and GSK3beta, where it is constitutively destined for proteasomal degradation as a result of constant phosphorylation by GSK3beta. Activation of the Wnt pathway results in stabilization and nuclear translocation of beta-catenin, resulting in beta-catenin target genes transcription. In addition to GSK3beta, PKCalpha is also reported to phosphorylate the Ser33/37/Thr41 residues of beta-catenin, and beta-catenin Ser45 is a known phosphorylation site for CK1alpha. In addition, HDAC6 and p300/CBP-associated factor (PCAF) regulate beta-catenin Lys49 acetylation.

Specificity

Recognizes active form of β-catenin, dephosphorylated on Ser37 or Thr41.
The antibody eptiope is 100% conserved in zebrafish.

Immunogen

Epitope: The epitope corresponds to amino acid residues 36-44 (HSGATTTAP) of human β-catenin.
Fusion protein corresponding to residues 1-100 of human β-catenin with a c-terminal histidine tag.

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Active-β-Catenin in NIH/3T3 cells.
Flow Cytometry Analysis: 0.1 µg of this antibody from a representative lot detected Active-β-Catenin in 1X10E6 A431 and HeLa cells.
The unconjugated version (Cat. No. 05-665) has been shown to work in WB, FC, ICC, IHC, IH(P).
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)
This Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor 647 is validated for use in Immunocytochemistry, Flow Cytometry for the detection of Active-β-Catenin.

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Active-β-Catenin in HeLa cells.

Target description

~85 kDa observed

Physical form

Protein G Purified
Purified mouse conjugate antibody in PBS with 15 mg/ml BSA and 0.1 % sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Yanchang Liu et al.
Frontiers in bioengineering and biotechnology, 9, 735949-735949 (2021-12-07)
Bone marrow mesenchymal stem cells (BMSCs) play a critical role in bone formation and are extremely sensitive to external mechanical stimuli. Mechanical signals can regulate the biological behavior of cells on the surface of titanium-related prostheses and inducing osteogenic differentiation

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