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05-419

Sigma-Aldrich

Anti-Myc Tag Antibody, clone 9E10

clone 9E10, Upstate®, from mouse

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Synonym(s):
Anti-CMyc
eCl@ss:
32160702
NACRES:
NA.43

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9E10, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

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This Item
06-54916-21305-724
vibrant-m

05-419

Anti-Myc Tag Antibody, clone 9E10

vibrant-m

06-549

Anti-Myc Tag Antibody

vibrant-m

05-724

Anti-Myc Tag Antibody, clone 4A6

clone

9E10, monoclonal

clone

polyclonal

clone

4A6, monoclonal

clone

4A6, monoclonal

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

ambient

UniProt accession no.

P01106

UniProt accession no.

P01106

UniProt accession no.

P01106

UniProt accession no.

P01106

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

western blot: suitable

technique(s)

ChIP: suitable (ChIP-seq), immunocytochemistry: suitable, immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable

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General description

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein′s biochemical properties.

The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.

Specificity

Recognizes Myc and recombinant proteins containing the Myc epitope tag.

Immunogen

Synthetic peptide corresponding to amino acids 408-437 of the Leucine zipper region of human Myc (AEEQKLISEEDLLRKRREQLKHKLEQLRNS).

Application

Anti-Myc Tag Antibody, clone 9E10 is an antibody against Myc Tag for use in IH(P), IC, IP & WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Quality

routinely evaluated by immunoblot on a Myc-tagged recombinant protein

Target description

Predicted MW for human Myc: 49 kDa

Physical form

Format: Purified
Protein G Purified
Protein G Purified immunoglobulin in 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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