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ROAMYC

Roche

Anti-c-myc

from mouse IgG1κ

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Synonym(s):
anti-myc

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9E10, monoclonal

assay

90% (SDS-PAGE)

form

frozen liquid (11667203001)
lyophilized (11667149001)

packaging

pkg of 200 μg (11667149001)
pkg of 5 mg (11667203001 [1 ml])

manufacturer/tradename

Roche

technique(s)

dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

epitope sequence

EQKLISEEDL

storage temp.

−20°C

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vibrant-m

ROAMYC

Anti-c-myc

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

purified immunoglobulin

clone

9E10, monoclonal

clone

9E10, monoclonal

clone

9E10, monoclonal

clone

9E10, monoclonal

isotype

IgG1κ

isotype

-

isotype

-

isotype

IgG1

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

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General description

Anti-c-myc is a monoclonal antibody to c-myc peptide (clone 9E10). Anti-c-myc recognizes the 9E10 epitope sequence (EQKLISEEDL), derived from the human c-myc protein. The monoclonal antibody against the c-myc epitope is well characterized and does not cross-react with other cellular proteins. The antibody recognizes its antigenic determinant even when the c-myc peptide epitope is introduced into unrelated recombinant proteins by a technique known as epitope tagging.
The cellular myelocytomatosis (c-myc) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The gene encoding it is mapped to human chromosome 8q24. It c-myc is a member of MYC gene family. c-Myc gene codes for basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor that regulates the G1-S cell cycle transition.


Contents:
  • 11 667 149 001: lyophilizate, stabilized
  • 11 667 203 001: frozen solution, (5 mg/ml)

Specificity

Anti-c-myc recognizes the amino acid sequence EQKLISEEDL, which was derived from the human c-myc protein. The antibody recognizes its antigenic determinant even when the c-myc peptide epitope is introduced into unrelated recombinant proteins either N-terminal, C-terminal or internal. The monoclonal antibody against the c-myc epitope is well characterized and does not crossreact with other cellular proteins.

Immunogen

Amino acid sequence EQKLISEEDL was derived from the human c-myc protein.

Application

Use Anti-c-myc for the detection of native human c-myc protein and recombinant epitope-tagged proteins that contain the c-myc epitope using:
  • Dot blots
  • Immunocytochemistry
  • Immunoprecipitation
  • Western blots
  • Immunohistochemistry.

Biochem/physiol Actions

The cellular myelocytomatosis (c-myc) oncogene has a crucial role in cellular proliferation, differentiation, apoptosis and acts as transcriptional regulator of gene expression. c-myc expression is essential and sufficient to assist most of the cells to enter DNA synthetic (S) phase of the cell cycle. The encoded protein plays a crucial role in vasculogenesis and angiogenesis during cancer development and progression. c-myc interacts with its binding partner Max and activates the transcription of growth promoting genes such as cyclin D2 and ornithine decarboxylase. It also represses the transcription of multiple genes, especially p21 and p27, by binding to the transcription initiator element (Inr) in a complex with Max and either Sp1 (specificity protein 1) or Miz1 (Myc-interacting zinc finger protein 1). Overexpression of MYC in DLBCL (diffuse large B-cell lymphoma) results in poor outcome and invasive treatment when medicated with rituximab plus cyclophosphamide, doxorubicin, vincristine and prednisone (R-CHOP).

Quality

Anti-c-myc antibody is tested for functionality and purity relative to a reference standard to confirm the quality of each new reagent lot.

Preparation Note

Working concentration: Working concentration of conjugate depends on application and substrate.

The following concentrations should be taken as a guideline:
  • Western blot: 1 to 10 μg/ml


Working solution: Tris-buffered saline containing 0.1% Tween 20.

Reconstitution

Add 500 μl PBS to a final concentration of 400 μg/ml.

Analysis Note

The monoclonal antibody against the c-myc epitope is well characterized and does not crossreact with other cellular proteins. No cross reaction with murine c-myc.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

11 - Combustible Solids

flash_point_f

does not flash

flash_point_c

does not flash


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Apoptotic signaling by c-MYC.
Hoffman B and Liebermann D A
Oncogene, 27(50), 6462-6472 (2008)
R Dalla-Favera et al.
Proceedings of the National Academy of Sciences of the United States of America, 79(24), 7824-7827 (1982-12-01)
Human sequences related to the transforming gene (v-myc) of avian myelocytomatosis virus (MC29) are represented by at least one gene and several related sequences that may represent pseudogenes. By using a DNA probe that is specific for the complete gene
Over-expression of the c-myc proto-oncogene in colorectal carcinoma.
Smith DR, et al.
British Journal of Cancer (1993)
HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity.
Gordan JD, et al.
Cancer Cell, 11(4), 335-347 (2007)
MAPK signal pathways in the regulation of cell proliferation in mammalian cells.
Cell Research, 12(1), 9-18 (2002)

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