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06-340

Sigma-Aldrich

Anti-Myc Antibody

Upstate®, from rabbit

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Synonym(s):
Transcription factor p64, avian myelocytomatosis viral oncogene homolog, myc proto-oncogene protein, v-myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian)
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse, Xenopus, avian

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

Gene Information

human ... MYC(4609)

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06-54916-213ABE25
Anti-Myc Antibody Upstate®, from rabbit

06-340

Anti-Myc Antibody

Anti-Myc Tag Antibody Upstate®, from rabbit

06-549

Anti-Myc Tag Antibody

Anti-acetyl-c-Myc (Lys148) Antibody from rabbit, purified by affinity chromatography

ABE25

Anti-acetyl-c-Myc (Lys148) Antibody

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

4A6, monoclonal

clone

polyclonal

species reactivity

human, mouse, Xenopus, avian

species reactivity

human

species reactivity

human

species reactivity

human, mouse

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

-

technique(s)

ChIP: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable

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General description

c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression.

Specificity

Recognizes Myc isoforms Mr 50-67kDa.
Will also cross-react with avian and Xenopus.

Immunogen

Bacterially expressed fusion-protein corresponding to the full-length human Myc.

Application

Immunoprecipitation:
4 µg of a previous antibody immuno-precipitated Myc as shown by an independent laboratory.

Immunocytochemistry:
This antibody has been used for immunocytochemistry by an independent laboratory. (Ohtsuka, T., 1998)
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Use Anti-Myc Antibody (rabbit polyclonal antibody) validated in ChIP, ICC, IP, WB to detect Myc also known as Transcription factor p64, avian myelocytomatosis viral oncogene homolog, myc proto-oncogene protein.

Quality

Routinely evaluated by western blot on A431 lysates from EGF-stimulated human A431 cells, human A431 cells and HeLa nuclear extract.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected Myc in A431 lysates from EGF-stimulated human A431 cells. 0.5-2 µg/mL of a previous lot detected Myc in RIPA lysates from human A431 cells and HeLa nuclear extract.

Target description

67 kDa

Physical form

Format: Purified
Immunoaffinity Chromatography
Purified rabbit polyclonal IgG in buffer containing PBS, 0.05% sodium azide with 2 mg/mL BSA. Frozen solution.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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c-Myc activation by Theileria parasites promotes survival of infected B-lymphocytes.
Dessauge, F; Hilaly, S; Baumgartner, M; Blumen, B; Werling, D; Langsley, G
Oncogene null
p19ARF directly and differentially controls the functions of c-Myc independently of p53.
Ying Qi, Mark A Gregory, Zhaoliang Li, Jeffrey P Brousal, Kimberly West, Stephen R Hann
Nature null
Proteomic profiling of Myc-associated proteins.
Agrawal, P; Yu, K; Salomon, AR; Sedivy, JM
Cell Cycle null
Tina Stoelzle et al.
BMC biology, 7, 63-63 (2009-09-30)
The oncoprotein c-Myc has been intensely studied in breast cancer and mouse mammary tumor models, but relatively little is known about the normal physiological role of c-Myc in the mammary gland. Here we investigated functions of c-Myc during mouse mammary
Lee A Campbell et al.
The FEBS journal, 284(23), 4096-4114 (2017-11-09)
Microglia, the resident macrophages of the brain, play a key role in the pathogenesis of HIV-associated neurocognitive disorders (HAND) due to their productive infection by HIV. This results in the release of neurotoxic viral proteins and pro-inflammatory compounds which negatively

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