06-549
Anti-Myc Tag Antibody
Upstate®, from rabbit
Synonym(s):
Transcription factor p64, avian myelocytomatosis viral oncogene homolog, myc proto-oncogene protein, v-myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian)
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
packaging
antibody small pack of 25 μg
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
General description
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein′s biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.
Specificity
Other species cross-reactivity not tested.
Recognizes and is specific for human Myc and recombinant proteins containing the Myc epitope tag.
Immunogen
Epitope: a.a. 409-420
Synthetic peptide corresponding to residues 409-420 of human Myc (MEQKLISEEDLN).
Application
Anti-Myc Tag Antibody is an antibody against Myc Tag for use in IC & WB.
Immunocytochemistry:
This antibody is reported to detect Myc-tagged proteins in transfected cells.
This antibody is reported to detect Myc-tagged proteins in transfected cells.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
Quality
Evaluated by Western Blot on Myc-PP2A A subunit transfected NIH/3T3 lysates.
Western Blotting:
1:500 dilution of this antibody detected myc-PP2A on 10 μg of Myc-PP2A A subunit transfected NIH/3T3 lysates.
Western Blotting:
1:500 dilution of this antibody detected myc-PP2A on 10 μg of Myc-PP2A A subunit transfected NIH/3T3 lysates.
Target description
Predicted molecular weight for human Myc: 49 kDa Obeserved molecular weight may vary.
Physical form
Format: Purified
Protein A chromatography
Purified rabbit in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution.
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
Analysis Note
Control
Positive control for human Myc: widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.
Positive control for human Myc: widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
recommended
Product No.
Description
Pricing
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Mxi1-SRalpha: a novel Mxi1 isoform with enhanced transcriptional repression potential.
Dugast-Darzacq, C; Pirity, M; Blanck, JK; Scherl, A; Schreiber-Agus, N
Oncogene null
Tubulin-targeting chemotherapy impairs androgen receptor activity in prostate cancer.
Zhu ML, Horbinski CM, Garzotto M, Qian DZ, Beer TM, Kyprianou N
Cancer Research null
V M Draviam et al.
The Journal of cell biology, 152(5), 945-958 (2001-03-10)
In this paper, we show that substrate specificity is primarily conferred on human mitotic cyclin-dependent kinases (CDKs) by their subcellular localization. The difference in localization of the B-type cyclin-CDKs underlies the ability of cyclin B1-CDK1 to cause chromosome condensation, reorganization
The zebrafish Iroquois gene iro7 positions the r4/r5 boundary and controls neurogenesis in the rostral hindbrain.
Lecaudey, V; Anselme, I; Rosa, F; Schneider-Maunoury, S
Development null
K Ojima et al.
The Journal of cell biology, 150(3), 553-566 (2000-08-10)
To learn how nebulin functions in the assembly and maintenance of I-Z-I bands, MYC- and GFP- tagged nebulin fragments were expressed in primary cultured skeletal myotubes. Their sites of incorporation were visualized by double staining with anti-MYC, antibodies to myofibrillar
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service