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06-549

Sigma-Aldrich

Anti-Myc Tag Antibody

Upstate®, from rabbit

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Synonym(s):
Transcription factor p64, avian myelocytomatosis viral oncogene homolog, myc proto-oncogene protein, v-myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian)
eCl@ss:
32160702
NACRES:
NA.43

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

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This Item
16-21305-419SAB2500663
vibrant-m

06-549

Anti-Myc Tag Antibody

vibrant-m

05-419

Anti-Myc Tag Antibody, clone 9E10

biological source

rabbit

biological source

mouse

biological source

mouse

biological source

goat

species reactivity

human

species reactivity

human

species reactivity

-

species reactivity

human, mouse, rat, canine

clone

polyclonal

clone

4A6, monoclonal

clone

9E10, monoclonal

clone

polyclonal

isotype

IgG

isotype

-

isotype

IgG1

isotype

-

NCBI accession no.

NM_002467.3

NCBI accession no.

NP_002458

NCBI accession no.

NM_002467.3

NCBI accession no.

-

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General description

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein′s biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.

Specificity

Other species cross-reactivity not tested.
Recognizes and is specific for human Myc and recombinant proteins containing the Myc epitope tag.

Immunogen

Epitope: a.a. 409-420
Synthetic peptide corresponding to residues 409-420 of human Myc (MEQKLISEEDLN).

Application

Anti-Myc Tag Antibody is an antibody against Myc Tag for use in IC & WB.
Immunocytochemistry:
This antibody is reported to detect Myc-tagged proteins in transfected cells.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Quality

Evaluated by Western Blot on Myc-PP2A A subunit transfected NIH/3T3 lysates.

Western Blotting:
1:500 dilution of this antibody detected myc-PP2A on 10 μg of Myc-PP2A A subunit transfected NIH/3T3 lysates.

Target description

Predicted molecular weight for human Myc: 49 kDa Obeserved molecular weight may vary.

Physical form

Format: Purified
Protein A chromatography
Purified rabbit in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Positive control for human Myc: widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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L Tian et al.
The Journal of biological chemistry, 275(11), 7854-7861 (2000-03-14)
The Wiskott-Aldrich syndrome is an inherited X-linked immunodeficiency characterized by thrombocytopenia, eczema, and a tendency toward lymphoid malignancy. Lymphocytes from affected individuals have cytoskeletal abnormalities, and monocytes show impaired motility. The Wiskott-Aldrich syndrome protein (WASP) is a multi-domain protein involved
K Ojima et al.
The Journal of cell biology, 150(3), 553-566 (2000-08-10)
To learn how nebulin functions in the assembly and maintenance of I-Z-I bands, MYC- and GFP- tagged nebulin fragments were expressed in primary cultured skeletal myotubes. Their sites of incorporation were visualized by double staining with anti-MYC, antibodies to myofibrillar
Mxi1-SRalpha: a novel Mxi1 isoform with enhanced transcriptional repression potential.
Dugast-Darzacq, C; Pirity, M; Blanck, JK; Scherl, A; Schreiber-Agus, N
Oncogene null
Tubulin-targeting chemotherapy impairs androgen receptor activity in prostate cancer.
Zhu ML, Horbinski CM, Garzotto M, Qian DZ, Beer TM, Kyprianou N
Cancer Research null
The zebrafish Iroquois gene iro7 positions the r4/r5 boundary and controls neurogenesis in the rostral hindbrain.
Lecaudey, V; Anselme, I; Rosa, F; Schneider-Maunoury, S
Development null

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