MilliporeSigma
All Photos(1)

Documents

16-219

Sigma-Aldrich

Anti-Myc Tag Antibody, clone 4A6, agarose conjugate

clone 4A6, Upstate®, from mouse

Sign Into View Organizational & Contract Pricing

eCl@ss:
32160702
NACRES:
NA.43

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4A6, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MYC(4609)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
16-21305-902R04-902
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

culture supernatant

Gene Information

human ... MYC(4609)

Gene Information

human ... MYC(4609)

Gene Information

-

Gene Information

-

species reactivity

human

species reactivity

human

species reactivity

-

species reactivity

vertebrates (common species)

clone

4A6, monoclonal

clone

4A6, monoclonal

clone

114-2C-7, monoclonal

clone

DW2, monoclonal

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

Specificity

Reactivity with other species has not been confirmed.

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc.

Application

Anti-Myc Tag Antibody, clone 4A6, agarose conjugate detects level of Myc Tag & has been published & validated for use in IP.
Immunoaffinity Purification:
A previous lot was used to purify Myc-tagged proteins. Elute with 100 mM tetraethyl ammonium (TEA), pH 11.5.

Features and Benefits

Format: Gel Immobilized

Quality

Routinely evaluated by immunoprecipitation of Myc-tagged Akt/PKB from a COS transfected cell lysate, subsequent detection using anti-Myc Tag (05-724).

Immunoprecipitation Analysis:
4 μg of anti-Myc Tag, clone 4A6, agarose conjugate immunoprecipitated Myc-tagged Akt/PKB from 250 μg of a COS transfected cell lysate, which was then detected by immunoblot analysis, using 0.5 μg/mL of anti-Myc Tag, clone 4A6 (Catalog # 05-724).

Target description

Varies

Physical form

Purified mouse monoclonal IgG1 covalently coupled to protein G agarose beads and provided as a 50% slurry in buffer containing PBS, pH 7.4, and 0.05% sodium azide. Liquid suspension.

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Cells transfected with Myc-tagged fusion vector.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Mutagenesis and analysis of genetic mutations in the GC-rich KISS1 receptor sequence identified in humans with reproductive disorders.
Luciana Madeira da Silva,Lauren Vandepas,Suzy D C Bianco
Journal of Visualized Experiments null
X Fu et al.
Journal of dental research, 96(12), 1451-1458 (2017-07-22)
Previous studies have identified the odd-skipped related 2 (Osr2) transcription factor as a key intrinsic regulator of palatal shelf growth and morphogenesis. However, little is known about the molecular program acting downstream of Osr2 in the regulation of palatogenesis. In
Dong-Lei Yang et al.
Cell discovery, 4, 55-55 (2018-10-23)
DNA methylation is a conserved epigenetic mark that is critical for many biological processes in plants and mammals. In Arabidopsis, the antagonistic activities of RNA-directed DNA methylation (RdDM) and ROS1-dependent active DNA demethylation are key for the dynamic regulation of
Tadashi Okubo et al.
Development (Cambridge, England), 138(2), 339-348 (2010-12-24)
The pharyngeal apparatus is a transient structure that gives rise to the thymus and the parathyroid glands and also contributes to the development of arteries and the cardiac outflow tract. A typical developmental disorder of the pharyngeal apparatus is the
Oluwatoyin E Akande et al.
Cell reports, 26(12), 3323-3335 (2019-03-21)
The control of p53 protein stability is critical to its tumor suppressor functions. The CREB binding protein (CBP) transcriptional co-activator co-operates with MDM2 to maintain normally low physiological p53 levels in cells via exclusively cytoplasmic E4 polyubiquitination activity. Using mass

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service