MilliporeSigma
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E6779

Millipore

EZview™ Red Anti-HA Affinity Gel

All Photos(1)
Synonym(s):
Anti-HA

shelf life

1 yr at 2‑8 °C

analyte chemical class(es)

proteins (hemagglutinin)

technique(s)

affinity chromatography: suitable
immunoprecipitation (IP): suitable

matrix

crosslinked agarose

capacity

≥0.4 mg/mL, gel binding capacity (HA-tagged fusion protein)

storage temp.

2-8°C

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EZview™ Red Anti-HA Affinity Gel

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AMDEX™ Sheep Anti Mouse IgG-HRP Cytiva RPN4201

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Anti-PECAM-1 Antibody, clone 2H8, Azide Free clone 2H8, Chemicon®, from hamster(Armenian)

Sigma-Aldrich

MAB1398Z

Anti-PECAM-1 Antibody, clone 2H8, Azide Free

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

technique(s)

-

technique(s)

-

technique(s)

electron microscopy: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, neutralization: suitable

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

-

shelf life

1 yr at 2‑8 °C

shelf life

-

shelf life

-

shelf life

-

analyte chemical class(es)

proteins (hemagglutinin)

analyte chemical class(es)

-

analyte chemical class(es)

-

analyte chemical class(es)

-

matrix

crosslinked agarose

matrix

-

matrix

-

matrix

-

capacity

≥0.4 mg/mL, gel binding capacity (HA-tagged fusion protein)

capacity

≥0.6 mg/mL, gel binding capacity

capacity

-

capacity

-

General description

EZview Red Anti-HA Affinity Gel is a red colored Anti-HA agarose affinity gel that contains anti-HA monoclonal antibody which is covalently attached to crosslinked agarose beads. The HA tag has the sequence YPYDVPDYA and derives from the hemagglutinin (HA) protein. This sequence occurs at amino acid residues 98-106 of the full-length HA protein. The HA tag has a high presence of charged amino acids, which enhances its likelihood of strong antibody recognition.

Specificity

Binding specificity: HA-tag peptide (N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C). It recognizes reduced forms of HA-tagged proteins and is reactive with N or C-terminal HA-tagged fusion proteins.

Application

EZview™ Red Anti-HA Affinity Gel has been used for immunoblot analysis and immunoprecipitation assay.
Suitable for Affinity capture (immunoprecipitation) of N- or C- terminal HA-tagged fusion proteins from E. coli or mammalian cell.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduces the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Features and Benefits

  • Increased visibility - Red color reduces risk of incidental aspiration
  • Improved recovery of target protein by reduced accidental loss
  • Higher reproducibility - More consistent yields

Other Notes

The affinity gel contains affinity purified anti-HA mouse monoclonal antibody (Clone HA-7) covalently attached to beaded agarose. The HA tag corresponds to amino acid residues 98-106 (YPYDVPDYA) of human influenza virus hemagglutinin (HA).

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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