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ROAHA

Roche

Anti-HA (12CA5)

from mouse IgG2bκ

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Synonym(s):
antibody

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

12CA5, monoclonal

Assay

>90% (HPLC and SDS-PAGE)

form

frozen liquid (11666606001)
lyophilized (11583816001)

packaging

pkg of 200 μg (11583816001)
pkg of 5 mg (11666606001 [1 ml])

manufacturer/tradename

Roche

concentration

5 mg/ml (11666606001)

isotype

IgG2bκ

epitope sequence

YPYDVPDYA

storage temp.

−20°C

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This Item
H3663ROAHAHASAB1306169
Anti-HA (12CA5) from mouse IgG2bκ

Roche

ROAHA

Anti-HA (12CA5)

Anti-HA High Affinity from rat IgG1

Roche

ROAHAHA

Anti-HA High Affinity

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

IgG fraction of antiserum

clone

12CA5, monoclonal

clone

HA-7, monoclonal

clone

3F10, monoclonal

clone

polyclonal

form

frozen liquid (11666606001), lyophilized (11583816001)

form

-

form

lyophilized

form

buffered aqueous solution

packaging

pkg of 200 μg (11583816001), pkg of 5 mg (11666606001 [1 ml])

packaging

-

packaging

pkg of 50 μg (11867423001), pkg of 500 μg (11867431001)

packaging

-

General description

Hemagglutinin HA is a dominant antigen present on the influenza viral surface. HA is a homotrimer, in which each monomer is produced as a single polypeptide. This monomer is cleaved into two subunits HA1 and HA2 by the host protease. The HA gene is mapped to segment 4 of influenza B viral genome.

Specificity

Anti-HA (12CA5) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).

Immunogen

Amino acids 98-106 from the human influenza virus hemagglutinin protein.

Application

Use Anti-HA (12CA5) for the detection of native influenza hemagglutinin protein and recombinant HA-tagged proteins using:
  • Dot blots
  • Immunochemistry
  • Immunoprecipitation
  • Western blotting
Note: For experiments in which sensitivity is not critical, use Anti-HA (12CA5). For higher sensitivity detection in western blotting at 10-fold lower concentration, use Anti-HA High Affinity (3F10).

Biochem/physiol Actions

Membrane fusion and receptor-binding action is the key function of hemagglutinin (HA) and it aids in viral entry and infection. HA is an influenza-virus glycoprotein that regulates membrane fusion and receptor-binding functions during viral entry and infection. The virus gains entry into the host cell via endocytosis and successive membrane fusion mediated by the HA antigen. HA plays a crucial role in viral pathogenesis and host response to viral infection.

Quality

Each lot of Anti-HA antibody is tested for immunoreactivity and purity relative to a reference standard.

Preparation Note

Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
  • Dot blot: 0.1 to 1 μg/ml
  • Immunfluorescence: 1-10 μg/ml
  • Western blot: 0.1 to 1 μg/ml

Reconstitution

11 583 816 001: Add 0.5 ml of PBS to a final concentration of 0.4 mg/ml.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

13 - Non Combustible Solids

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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