MilliporeSigma
All Photos(1)

Documents

H6533

Sigma-Aldrich

Anti-HA−Peroxidase antibody, Mouse monoclonal

clone HA-7, purified from hybridoma cell culture

Sign Into View Organizational & Contract Pricing

Synonym(s):
Monoclonal Anti-HA, Anti-HA, Anti-Influenza Hemagglutinin
MDL number:
NACRES:
NA.56

biological source

mouse

Quality Level

conjugate

peroxidase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HA-7, monoclonal

form

lyophilized powder

packaging

vial of 0.5 mL

concentration

5-11 mg/mL

technique(s)

western blot: 1:4,000 using using HA tagged fusion protein expressed in bacteria, and ECL immunoblotting detection reagent

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
A5477H3663H6908
vibrant-m

H3663

Anti-HA antibody, Mouse monoclonal

vibrant-m

H6908

Anti-HA antibody produced in rabbit

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

unconjugated

conjugate

unconjugated

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

technique(s)

western blot: 1:4,000 using using HA tagged fusion protein expressed in bacteria, and ECL immunoblotting detection reagent

technique(s)

western blot: 1:4,000 using extracts of mammalian cells expressing HA tagged fusion proteins

technique(s)

immunocytochemistry: 1.0-2.0 μg/mL, immunoprecipitation (IP): 0.5-2.0 μg, western blot: 0.25-1 μg/mL

technique(s)

immunoprecipitation (IP): 2.5-4 μg/test using HA-tagged fusion protein from cell lysates, indirect immunofluorescence: 10-20 μg/mL using HA-tagged fusion protein transfected cells, western blot: 0.5-0.8 μg/mL using HA-tagged fusion protein transfected cell extracts

form

lyophilized powder

form

buffered aqueous glycerol solution

form

-

form

buffered aqueous solution

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Influenza A virus, a RNA virus, encodes two surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA). HA stalk domain based epitope region antibody is effective in neutralizing divergent influenza virus strains.
The mouse monoclonal Anti-HA-Peroxidase antibody recognizes native and denatured-reduced forms of the HA-tagged proteins. It also reacts with the N- or C-terminal HA-tagged fusion proteins expressed in E. coli and mammalian cells.

Specificity

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

Immunogen

synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH

Application

Monoclonal Anti-HA-Peroxidase antibody produced in mouse has been used:
  • in immunopreciptation and immunostaining of human member 9 of solute carrier family 38 SLC38A9 transfected in HeLa cells
  • in immunoblot detection in transfected A375 cells
  • is suitable for immunoblotting of nucleosome assembly protein NAP1L1

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.

Preparation Note

Reconstitute in .5mL of distilled water.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Joanna F McGouran et al.
Chemistry & biology, 20(12), 1447-1455 (2013-12-03)
Posttranslational modification with ubiquitin (Ub) controls many cellular processes, and aberrant ubiquitination can contribute to cancer, immunopathology, and neurodegeneration. The versatility arises from the ability of Ub to form polymer chains with eight distinct linkages via lysine side chains and
Ernest C So et al.
The Journal of biological chemistry, 291(11), 5832-5843 (2016-01-13)
Legionella pneumophila, the causative agent of Legionnaire's disease, uses its type IV secretion system to translocate over 300 effector proteins into host cells. These effectors subvert host cell signaling pathways to ensure bacterial proliferation. Despite their importance for pathogenesis, the
Hepatitis C virus NS5A targets nucleosome assembly protein NAP1L1 to control the innate cellular response
Cevik RE, et al.
Journal of virology, 91(18), e00880-e00817 (2017)
miR-216b enhances the efficacy of vemurafenib by targeting Beclin-1, UVRAG and ATG5 in melanoma
Luo M, et al.
Cellular Signalling, 42, 30-43 (2018)
Katharina L Willmann et al.
Nature communications, 5, 5360-5360 (2014-11-20)
Primary immunodeficiency disorders enable identification of genes with crucial roles in the human immune system. Here we study patients suffering from recurrent bacterial, viral and Cryptosporidium infections, and identify a biallelic mutation in the MAP3K14 gene encoding NIK (NF-κB-inducing kinase).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service