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05-736

Sigma-Aldrich

Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone SK703, rabbit monoclonal

clone SK703, Upstate®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

SK703, monoclonal

species reactivity

human, mouse

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pSer473)

Gene Information

human ... AKT1(207)

Specificity

phosphorylated Akt1/PKBa

Immunogen

Peptide containing a pSer that corresponds to amino acid position 473 of human Akt1/PKBa

Application

Detect phospho-Akt1/PKBα (Ser473) using this Anti-Akt1 Antibody, clone SK703 validated for use in WB.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling

Quality

routinely evaluated by immunoblot on lysates from mouse NIH-3T3 fibroblasts treated with PDGF

Target description

60kDa

Physical form

Format: Purified
Protein A purified
Protein A purified antibody in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide.

Storage and Stability

Stable refrigerated at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Matthew J Nelson et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 300(4), R993-R1000 (2011-02-11)
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BMC cancer, 15, 990-990 (2015-12-23)
Retinoblastoma protein-interacting zinc-finger gene 1 (RIZ1) displays strong tumor suppressive activities, and its expression is often silenced in many types of human tumors. However, the relationship between RIZ1 expression and glioma prognosis remains unclear. The dysregulation of RIZ1 was evaluated
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Clinical cancer research : an official journal of the American Association for Cancer Research, 17(4), 762-770 (2010-11-26)
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Mitch T Sitnick et al.
Diabetes, 62(10), 3350-3361 (2013-07-10)
Intramyocellular triacylglycerol (IMTG) accumulation is highly associated with insulin resistance and metabolic complications of obesity (lipotoxicity), whereas comparable IMTG accumulation in endurance-trained athletes is associated with insulin sensitivity (the athlete's paradox). Despite these findings, it remains unclear whether changes in

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