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07-276

Sigma-Aldrich

Anti-PDGFRα Antibody

Upstate®, from rabbit

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Synonym(s):
Anti-Anti-CD140A, Anti-Anti-PDGFR-2, Anti-Anti-PDGFR2
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse

packaging

antibody small pack of 25 μL

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... PDGFRA(5156)

Related Categories

General description

Platelet derived growth factor receptor (PDGF Receptor) is a transmembrane glycoprotein of 170-185 kDa which undergoes homo or heterodimerization into complexes of alpha and beta subunits upon ligand binding, depending on the isoform of PDGF (PDGF AA, BB or AB) that binds. The phosphorylation of tyrosine residues in the now activated receptor can control multiple signaling events such as actin reorganization, transcription, cell growth, migration and differentiation, and also lead to activation of the Ras, Raf and ERK 1 and 2 pathway.

Specificity

Recognizes PDGF Receptor alpha.

Immunogen

peptide corresponding to amino acids 1035-1053 (C-GKRNRHSSQTSEESAIETG) of human PDGF Receptor α

Application

Detect PDGFRα using this Anti-PDGFRα Antibody validated for use in IP & WB.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors

Quality

routinely evaluated by immunoblot on 3T3/NIH cell lysate

Target description

150/170 kDa (doublet)

Physical form

ImmunoAffinity Purified
Protein A Purified immunoglobulin in 0.14 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HeLa cells, extracts from 3T3/NIH or HepG2 cells, human brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Evidence for PDGFRA, PDGFRB and KIT deregulation in an NSCLC patient.
T Negri et al.
British journal of cancer, 96(1), 180-181 (2007-01-11)
Enhanced transduction and replication of rGD-fiber Modified adenovirus in primary t cells.
Sengupta S, Ulasov IV, Thaci B, Ahmed AU, Lesniak MS
Testing null
Activity of AMN107, a novel aminopyrimidine tyrosine kinase inhibitor, against human FIP1L1-PDGFR-alpha-expressing cells.
Srdan Verstovsek, Francis J Giles, Alfonso Quintas-Cardama, Taghi Manshouri, Ly Huynh et al.
Leukemia Research null
The novel tyrosine kinase inhibitor EXEL-0862 induces apoptosis in human FIP1L1-PDGFR-alpha-expressing cells through caspase-3-mediated cleavage of Mcl-1.
Pan, J; Quintas-Cardama, A; Manshouri, T; Giles, FJ; Lamb, P; Tefferi, A; Cortes et al.
Leukemia null
PDGF- and insulin/IGF-1-specific distinct modes of class IA PI 3-kinase activation in normal rat retinas and RGC-5 retinal ganglion cells.
Biswas, SK; Zhao, Y; Nagalingam, A; Gardner, TW; Sandirasegarane, L
Investigative Ophthalmology & Visual Science null

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