07-981
Anti-Cbx7 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
chromobox homolog 7, chromobox protein homolog 7
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CBX7(23492)
Related Categories
General description
Chromobox protein homologue 7 (Cbx7) is a member of the mammalian chromobox family of proteins. As a component of the Polycomb repressive complex 1 (PRC1), Cbx7, is involved in the maintenance and prolongation of cultured epithelial cell lifespan, and has been shown to function independently of BMI-1 in the repression of INK4a/ARF, a known tumor suppressor locus. Cbx7 is also able to regulate cellular lifespan through maintaining CDKN2A repression. Studies have shown Cbx7 to be up-regulated in follicular lymphoma, suggesting potential as a human oncogene.
Specificity
This antibody recognizes Cbx7 at the C-terminus.
Immunogen
Epitope: C-terminus
GST-tagged recombinant protein corresponding to the C-terminus of mouse Cbx7.
Application
Anti-Cbx7 Antibody is a Rabbit Polyclonal Antibody for detection of Chromobox protein homolog 7 (Cbx7) also known as hromobox protein homolog 7 & has been validated in WB & IP.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Western Blot (Snap i.d.) Analysis: 2 µg/mL from a representative lot detected Cbx7 on 10 µg of human and mouse embryonic stem cell lysates.
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP.
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP.
Quality
Evaluated by Western Blot in mouse embryonic stem cell lysate.
Western Blot Analysis: 0.25 µg/mL of this antibody detected Cbx7 on 10 µg of mouse embryonic stem cell lysate.
Western Blot Analysis: 0.25 µg/mL of this antibody detected Cbx7 on 10 µg of mouse embryonic stem cell lysate.
Target description
~21 kDa observed. A non-specific band at ~ 44 kDa and may be observed in some lysates.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse embryonic stem cell lysate
Mouse embryonic stem cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Elizabeth S Jaensch et al.
Molecular cell, 81(22), 4677-4691 (2021-10-13)
The CBX family of proteins is central to proper mammalian development via key roles in Polycomb-mediated maintenance of repression. CBX proteins in differentiated lineages have chromatin compaction and phase separation activities that might contribute to maintaining repressed chromatin. The predominant
Eleanor Glancy et al.
Molecular cell, 83(9), 1393-1411 (2023-04-09)
Polycomb repressive complex 2 (PRC2) mediates H3K27me3 deposition, which is thought to recruit canonical PRC1 (cPRC1) via chromodomain-containing CBX proteins to promote stable repression of developmental genes. PRC2 forms two major subcomplexes, PRC2.1 and PRC2.2, but their specific roles remain
Junghyun L Suh et al.
Cell chemical biology, 29(4), 555-571 (2021-10-30)
Canonical targeting of Polycomb repressive complex 1 (PRC1) to repress developmental genes is mediated by cell-type-specific, paralogous chromobox (CBX) proteins (CBX2, 4, 6, 7, and 8). Based on their central role in silencing and their dysregulation associated with human disease
Nadezda A Fursova et al.
Molecular cell, 74(5), 1020-1036 (2019-04-29)
The Polycomb system modifies chromatin and plays an essential role in repressing gene expression to control normal mammalian development. However, the components and mechanisms that define how Polycomb protein complexes achieve this remain enigmatic. Here, we use combinatorial genetic perturbation
Jin He et al.
Nature cell biology, 15(4), 373-384 (2013-03-19)
Polycomb group (PcG) proteins play important roles in repressing lineage-specific genes and maintaining the undifferentiated state of mouse embryonic stem cells (mESCs). However, how PcG proteins are recruited to their target genes is largely unknown. Here, we show that the
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