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MAB3118

Sigma-Aldrich

Anti-DYKDDDDK (FLAG® epitope tag) Antibody, clone 2EL-1B11

ascites fluid, clone 2EL-1B11, Chemicon®

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eCl@ss:
32160702
NACRES:
NA.43

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

2EL-1B11, monoclonal

species reactivity

vertebrates, E. coli

species reactivity (predicted by homology)

rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

shipped in

dry ice

target post-translational modification

unmodified

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This Item
F2555MAB3678AB3254
species reactivity

vertebrates, E. coli

species reactivity

-

species reactivity

E. coli, vertebrates

species reactivity

vertebrates

clone

2EL-1B11, monoclonal

clone

SIG1-25, monoclonal

clone

CAT-1, monoclonal

clone

polyclonal

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

chicken

isotype

IgG1

isotype

IgG

isotype

IgG1

isotype

-

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

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General description

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

FLAG® is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Specificity

DYKDDDDK sequence containing proteins. Reacts with both N-terminal and C-terminal fusion proteins.

Immunogen

Epitope: Epitope tag
Synthetic peptide M-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys(C) coupled to ovalbumin

Application

Detect DYKDDDDK (FLAGepitope tag) using this Anti-DYKDDDDK (FLAG epitope tag) Antibody, clone 2EL-1B11 validated for use in ELISA, IC, IF, IP & WB.
Immunofluorescence:
A 1:1,000-1:5,000 dilution of a previous lot was used in IF.

Immunoprecipitation:
A 1:1,000-1:5,000 dilution of a previous lot was used IP on recombinant proteins.

ELISA:
A 1:1,000-1:5,000 dilution of a previous lot was used in Enzyme-linked immunosorbent assay on recombinant proteins.
Note: Does not require Ca++ for binding.

Immunoblotting:
on recombinant proteins

Optimal working dilutions must be determined by the end user.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Quality

Routinely evaluated by Western Blot on Flag-mTor protein blot.

Western Blot Analysis:
1:500 dilution of this lot detected Flag mTor on Flag-mTOR blot.

Linkage

Replaces: AB3250

Physical form

Ascites mouse monoclonal IgG1 liquid containing no preservative.
Unpurified

Storage and Stability

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Cells transfected with FLAG-tagged fusion vector

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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