09-078
Anti-EHMT1/GLP1 Antibody
Upstate®, from rabbit
Synonym(s):
G9a like protein, H3-K9-HMTase, Histone H3-K9 methyltransferase, Histone-lysine N-methyltransferase, H3 lysine-9 specific, EC 2.1.1.43, euchromatic histone methyltransferase 1, euchromatic histone-lysine N-methyltransferase 1, Eu-HMTase1, FLJ12879 1, FP1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... EHMT1(79813)
General description
G9a-Like Protein 1 (GLP1), also known as Euchromatic histone lysine N-methyl-transferase 1 (EMHT1), is a histone methyltransferase that methylates Lys9 of histone H3. H3Lys9 methylation is a marker for epigenetic transcriptional repression. EMHT1/GLP1 is targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it contributes to silencing of Myc- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. EMHT1/GLP1 is phosphorylated upon DNA damage, probably by ATM or ATR. Defects in EHMT1 result in severe mental retardation, hypotonia, and epileptic seizures.
Specificity
Recognizes EMHT1/GLP1
Immunogen
Epitope: a.a. 40-59
KLH conjugated synthetic peptide (KHTQDSARVNPQDGTNTLTR(C) corresponding to a.a. 40-59 of human EMHT1/GLP1.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Histones
Chromatin Biology
Histones
Use Anti-EHMT1/GLP1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect EHMT1/GLP1 also known as H3-K9-HMTase 5, Histone H3-K9 methyltransferase 5.
Quality
Routinely evaluated by immunoblot.
Target description
160 kda
Physical form
ImmunoAffinity Purified
Immunoaffinity purified rabbit IgG in PBS containing 0.1% sodium azide, 30% glycerol
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Dan Levy et al.
Nature immunology, 12(1), 29-36 (2010-12-07)
Signaling via the methylation of lysine residues in proteins has been linked to diverse biological and disease processes, yet the catalytic activity and substrate specificity of many human protein lysine methyltransferases (PKMTs) are unknown. We screened over 40 candidate PKMTs
Coralie Poulard et al.
Cell death & disease, 9(10), 1038-1038 (2018-10-12)
Synthetic glucocorticoids (GCs) are used to treat lymphoid cancers, but many patients develop resistance to treatment, especially to GC. By identifying genes that influence sensitivity to GC-induced cell death, we found that histone methyltransferases G9a and G9a-like protein (GLP), two
Rui Wang et al.
Journal of the American Chemical Society, 135(3), 1048-1056 (2012-12-19)
Protein methyltransferases (PMTs) have emerged as important epigenetic regulators in myriad biological processes in both normal physiology and disease conditions. However, elucidating PMT-regulated epigenetic processes has been hampered by ambiguous knowledge about in vivo activities of individual PMTs particularly because
Coralie Poulard et al.
EMBO reports, 18(8), 1442-1459 (2017-06-16)
Like many transcription regulators, histone methyltransferases G9a and G9a-like protein (GLP) can act gene-specifically as coregulators, but mechanisms controlling this specificity are mostly unknown. We show that adjacent post-translational methylation and phosphorylation regulate binding of G9a and GLP to heterochromatin
Xiaopeng Lu et al.
Nucleic acids research, 47(21), 10977-10993 (2019-10-16)
The binding of p53-binding protein 1 (53BP1) to damaged chromatin is a critical event in non-homologous DNA end joining (NHEJ)-mediated DNA damage repair. Although several molecular pathways explaining how 53BP1 binds damaged chromatin have been described, the precise underlying mechanisms
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