MilliporeSigma
All Photos(1)

Documents

G2654

Sigma-Aldrich

Monoclonal Anti-Glucagon antibody produced in mouse

clone K79bB10, ascites fluid

Sign Into View Organizational & Contract Pricing

Synonym(s):
Glucagon Antibody, Glucagon Antibody - Monoclonal Anti-Glucagon antibody produced in mouse
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

K79bB10, monoclonal

contains

15 mM sodium azide

species reactivity

feline, canine, mouse, guinea pig, rat, rabbit, human, pig

technique(s)

dot blot: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:2,000 using human or animal pancreas
radioimmunoassay: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GCG(2641)
mouse ... Gcg(14526)
rat ... Gcg(24952)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SAB4200685I2018D8168
conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

unconjugated

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

ascites fluid

clone

K79bB10, monoclonal

clone

K79bB10, monoclonal

clone

K36AC10, monoclonal

clone

MANDYS8, monoclonal

species reactivity

feline, canine, mouse, guinea pig, rat, rabbit, human, pig

species reactivity

cat, rabbit, mouse, guinea pig, rat, dog, porcine, zebrafish, human

species reactivity

feline, human, rat, horse, rabbit, guinea pig, bovine, monkey, sheep, canine, pig

species reactivity

chicken, rat, human, pig, rabbit, mouse

technique(s)

dot blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:2,000 using human or animal pancreas, radioimmunoassay: suitable

technique(s)

immunohistochemistry: 5-10 μg/mL using heat−retrieved formalin-fixed, paraffin-embedded Rat pancreas sections and Biotin/ExtrAvidin®-Peroxidase staining system., radioimmunoassay: suitable

technique(s)

dot blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human or animal pancreas, radioimmunoassay: suitable

technique(s)

indirect ELISA: suitable, indirect immunofluorescence: 1:400 using frozen human or animal muscle tissue sections., microarray: suitable, western blot: suitable

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Glucagon is a 29 amino acid peptide hormone, which is produced by alpha cells of the pancreas. It is a hyperglycemic hormone. The gene is located on human chromosome 2q24.2.

Specificity

Monoclonal Anti-Glucagon reacts with pancreatic glucagon in RIA and immunocytochemistry. The affinity constant of 6.1 x 10(8) L/M in RIA. The antibody weakly cross-reacts with gut glucagon (enteroglucagon) in an immunohistological assay. Cross-reactivity has been observed with glucagon-containing cells in fixed sections of pancreas from human, porcine, dog, rabbit, mouse, rat, guinea pig, and cat.
The antibody reacts specifically against pancreatic glucagon and exhibits only very weak cross-reaction with gut glucagon (enteroglucagon). May be used for the immunohistochemical staining of Bouin′s-fixed, and formalin-fixed, paraffin-embedded pancreatic tissue sections. Binds to glucagon with an affinity constant of 6.1 × 108 M-1 in RIA.
Monoclonal anti-Glucagon antibody can be used as an analytical tool for quantification of the hormone. It can also be used for immunocytochemical staining of formalin fixed and Bouin-fixed, paraffin-embedded pancreatic tissue sections. Mouse anti-Glucagon antibody reacts specifically with pancreatic glucagon. The product has also shown cross reactivity with glucagon-containing cells in fixed sections of pancreas from dog, mouse, rat, rabbit, porcine, guinea pig, cat and human and weak cross reactivity for gut glucagon (enteroglucagon).
The product is provided as ascites fluid containing 0.1% sodium azide as a preservative.

Immunogen

Polymerized porcine glucagon.

Application

Monoclonal Anti-Glucagon antibody produced in mouse has been used:
  • for the immunostaining of pancreatic tissue
  • in flow cytometry and immunofluorescence imaging of pancreas cells
  • for immunohistochemistry and morphology of pancreas

Biochem/physiol Actions

Glucagon is a 29-residue polypeptide hormone (MW 3482), produced in the pancreas. A related hormone, enteroglucagon (or oxyntomodulin), which is produced in the mucosa of the small and large intestine, consists of the 29 amino acid sequence of pancreatic glucagons extended by 8 additional residues at the C-terminus. The biological activities of pancreatic glucagon include glycogenolysis, lipolysis, gluconeogenesis, and ketogenesis, which are antagonistic effects to those of insulin action, thus leading to increased blood glucose levels.. Glucagon regulates plasma lipid levels, energy balance and food intake. Glucagon and insulin have opposing roles and control glucose levels. Glucagon suppression is considered as one of the many therapies for controlling diabetes. Immunocytochemical studies have revealed the presence of pancreatic glucagon inside the A or cells, which constitute 15-20% of the islet cell population. These cells are located preferentially at the periphery of the human pancreatic islets. Glucagon imbalance could also contribute to hyperglycemia in diabetes mellitus. Deletion in the glucagon gene locus is associated with mental retardation and facial dysmorphism in children and infants. Pathological manifestations of the glucagon-type peptide reside almost exclusively with the existence of tumors or glucagonomas, as no states of glucagon-cell deficiency or hyperplasia have been identified. High levels of glucagon is associated with pancreatic tumors and necrolytic migratory erythema.
Glucagon-specific antibodies would prove useful as an a cell and tumor markers applying immunohistochemical techniques, and as an analytical tool in quantification of the hormone.
Glucagon is critical in increasing blood sugar through glycogenolysis and increased gluconeogenesis. It regulates lipid and protein metabolism and modulates the fuel supply to all the organs. Glucagon plays a vital role in the modulation of postprandial hepatic glucose production. It is used to treat obesity. Elevated levels of glucagon are associated with glucagonoma and type 1 diabetes. It acts as a ligand to specific G-protein coupled receptor.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 2

1 of 2

Glucagon synthetic, powder, suitable for cell culture

Sigma-Aldrich

G2044

Glucagon

Isolation and purification of rat islet cells by flow cytometry.
Akbarzadeh A, et al.
Indian Journal of Clinical Biochemistry : IJCB, 23(1), 57-57 (2008)
Glucagonoma syndrome: a review and update on treatment.
John A M and Schwartz R A
Journal of the European Academy of Dermatology and Venereology : JEADV, 30(12), 2016-2022 (2016)
The metabolic actions of glucagon revisited.
Habegger K M, et al.
Nature reviews. Endocrinology, 6(1), 689-689 (2010)
Glucagon and amino acids are linked in a mutual feedback cycle: the liver-alpha-cell axis
Holst JJ, et al.
Diabetes, 66(2), 235-240 (2017)
Point mutations in the PDX1 transactivation domain impair human beta-cell development and function
Wang X, et al.
Molecular Metabolism (2019)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service