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MABN238

Sigma-Aldrich

Anti-Glucagon Antibody, clone 13D11.33

clone 13D11.33, from mouse

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Synonym(s):
4030-01F, 4031-01F, AB932
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

13D11.33, monoclonal

species reactivity

human, rat

technique(s)

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
multiplexing: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GCG(2641)

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This Item
SAB4501137WH0002641M1G2654
clone

13D11.33, monoclonal

clone

polyclonal

clone

2D3-2B11, monoclonal

clone

K79bB10, monoclonal

species reactivity

human, rat

species reactivity

human, rat, mouse

species reactivity

human

species reactivity

feline, canine, mouse, guinea pig, rat, rabbit, human, pig

Gene Information

human ... GCG(2641)

Gene Information

human ... GCG(2641)

Gene Information

human ... GCG(2641)

Gene Information

human ... GCG(2641)
mouse ... Gcg(14526)
rat ... Gcg(24952)

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

ascites fluid

isotype

IgG2bκ

isotype

-

isotype

IgG1κ

isotype

IgG1

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General description

Glucagon is a hormone that is proteolytically cleaved into at least eight different functional peptides. It is produced by pancreatic cells and it has binding sites in the brain, intestines, and liver, among other tissues. Glucagon is secreted in response to hypoglycemia. Through the combined activation of Gsα- and Gq-coupled transmembrane receptors and their downstream signaling pathways, glucagon stimulates the release of glucose from the liver into the bloodstream. One important mechanism underlying this process is the activation of protein kinase A (PKA). PKA signals result in increased transcription of phosphoenolpyruvate carboxykinase, which encodes the rate-limiting enzyme in the production of glucose from noncarbohydrate substrates. PKA signals may also increase the breakdown of glycogen, and suppress glycolysis. Glucagon may also engage in calcium-dependent signaling via PLC. The importance of glucagon in regulating glucose homeostasis, in conjunction with insulin, is underscored by findings that glucagon and glucagon receptor signaling are disrupted in type II diabetes.

Immunogen

Ovalbumin-conjugated linear peptide corresponding to human Glucagon.

Application

Detect Glucagon using this Anti-Glucagon Antibody, clone 13D11.33 validated for use in IHC(P), Immunofluorescence, ELISA, Multiplexing.
Immunohistochemistry Analysis: A 1:8,000 dilution from a representative lot detected Glucagon in normal rat pancreas tissue (For enhanced staining results, it is recommended to use a 10,000 dilution of this antibody.).

ELISA Analysis: A representative lot detected Glucagon in ELISA.

Luminex® Analysis: A representative lot detected Glucagon in Luminex Assay.
Research Category
Neuroscience
Research Sub Category
Sensory & PNS

Quality

Evaluated by Immunofluorescence in normal rat pancreas tissue.

Immunofluorescence Analysis: A 1:8,000 dilution of this antibody detected Glucagon in normal rat pancreas tissue.

Target description

21 kDa calculated

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Normal rat pancreas tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

Luminex is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Karin J Bosma et al.
Molecular metabolism, 54, 101347-101347 (2021-10-10)
Type 2 diabetes is characterized by hyperglycemia and inflammation. Prostaglandin E2, which signals through four G protein-coupled receptors (EP1-4), is a mediator of inflammation and is upregulated in diabetes. We have shown previously that EP3 receptor blockade promotes β-cell proliferation
Chandan Sona et al.
JCI insight, 7(6) (2022-02-09)
BACKGROUNDPathophysiology of type 1 diabetes (T1D) is illustrated by pancreatic islet infiltration of inflammatory lymphocytes, including CD8+ T cells; however, the molecular factors mediating their recruitment remain unknown. We hypothesized that single-cell RNA-sequencing (scRNA-Seq) analysis of immune cell populations isolated
Peter A Kropp et al.
American journal of physiology. Endocrinology and metabolism, 314(4), E308-E321 (2018-01-20)
The transcription factors pancreatic and duodenal homeobox 1 (Pdx1) and onecut1 (Oc1) are coexpressed in multipotent pancreatic progenitors (MPCs), but their expression patterns diverge in hormone-expressing cells, with Oc1 expression being extinguished in the endocrine lineage and Pdx1 being maintained
Jing Liu et al.
Developmental cell, 48(1), 49-63 (2019-01-09)
In the developing pancreas, transient Neurog3-expressing progenitors give rise to four major islet cell types: α, β, δ, and γ; when and how the Neurog3+ cells choose cell fate is unknown. Using single-cell RNA-seq, trajectory analysis, and combinatorial lineage tracing
Gabriel Cao et al.
PloS one, 16(6), e0243340-e0243340 (2021-06-12)
The objective of this work was to analyze the structural changes of the pancreatic islets in rats, after 6 month consuming regular and light cola for 6 months. Also, we have analyzed the possible role of PDX-1 in that process.

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