MilliporeSigma
All Photos(3)

Documents

I2018

Sigma-Aldrich

Monoclonal Anti-Insulin antibody produced in mouse

clone K36AC10, ascites fluid

Sign Into View Organizational & Contract Pricing

Synonym(s):
Insulin Antibody, Insulin Antibody - Monoclonal Anti-Insulin antibody produced in mouse
MDL number:
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

K36AC10, monoclonal

contains

15 mM sodium azide

species reactivity

feline, human, rat, horse, rabbit, guinea pig, bovine, monkey, sheep, canine, pig

technique(s)

dot blot: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human or animal pancreas
radioimmunoassay: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... INS(3630)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SAB4200691G2654D8168
conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

unconjugated

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

ascites fluid

clone

K36AC10, monoclonal

clone

K36AC10, monoclonal

clone

K79bB10, monoclonal

clone

MANDYS8, monoclonal

species reactivity

feline, human, rat, horse, rabbit, guinea pig, bovine, monkey, sheep, canine, pig

species reactivity

guinea pig, porcine, bovine (proinsulin), rat, monkey, sheep, dog, horse, human (insulin ), rabbit

species reactivity

feline, canine, mouse, guinea pig, rat, rabbit, human, pig

species reactivity

chicken, rat, human, pig, rabbit, mouse

technique(s)

dot blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human or animal pancreas, radioimmunoassay: suitable

technique(s)

immunoblotting: suitable, immunocytochemistry: suitable, immunofluorescence: 2.5-5 μg/mL using ß -TC-6 Mouse Embryo Pancrease Insulinoma cells., immunohistochemistry: 10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human pancreas sections and Biotin/ExtrAvidin®-Peroxidase staining system., radioimmunoassay: suitable

technique(s)

dot blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:2,000 using human or animal pancreas, radioimmunoassay: suitable

technique(s)

indirect ELISA: suitable, indirect immunofluorescence: 1:400 using frozen human or animal muscle tissue sections., microarray: suitable, western blot: suitable

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

The antibody reacts specifically against insulin by RIA and immunocytochemistry. It exhibits cross-reactivity with human proinsulin. The antibody binds to insulin with an affinity constant of 8.8 × 109 M-1 in RIA.
The insulin gene is mapped to human chromosome 11p15.5.

Application

Monoclonal Anti-Insulin antibody produced in mouse has been used in immunoblotting assay and ELISA.
Monoclonal Anti-Insulin antibody produced in mouse was used in ELISA-based protein pin array assay to study the interactions between human αB crystalline and insulin. It was used for flow cytometric analysis of rat white blood cells and mast cells.

Biochem/physiol Actions

Insulin is a major regulatory hormone important in the metabolic processes, transport of glucose, amino acids and certain ions. It is also involved in the synthesis of nucleic acids and proteins. The biological effects of insulin are mediated by insulin receptor and it integrates multiple major signaling pathways.
Insulin is a major regulatory hormone important in the metabolic processes, transport of glucose, amino acids and certain ions. It is also involved in the synthesis of nucleic acids and proteins. The biological effects of insulin are mediated by insulin receptor and it integrates multiple major signaling pathways. Abnormal insulin secretion or function leads to diabetes mellitus, obesity and other carbohydrate metabolism disorders. Insulin is known to stimulate cell division.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

H Keilacker et al.
Biomedica biochimica acta, 45(8), 1093-1102 (1986-01-01)
We investigated four insulin-specific hybridoma antibodies with respect to their kinetic properties as well as the binding behaviour of some combinations of them. From equilibrium binding data all but one antibodies were shown to bear homogeneous binding sites. They revealed
Sandra K Szlapinski et al.
The Journal of physiology, 597(16), 4237-4250 (2019-06-18)
Pancreatic β-cell dysfunction is hypothesized to be the significant determinant of gestational diabetes pathogenesis, however pancreatic samples from patients are scarce. This study reports a novel mouse model of gestational glucose intolerance in pregnancy, originating from previous nutrition restriction in
Proinsulin-specific monoclonal antibodies. Immunocytochemical application as beta-cell markers and as probes for conversion.
O D Madsen
Diabetes, 36(10), 1203-1211 (1987-10-01)
Xiao-Yi Zeng et al.
PloS one, 7(7), e42115-e42115 (2012-08-04)
The present study investigated the chronic efficacy of oleanolic acid (OA), a triterpenoid selected from our recent screening, on hyperglycemia in type-2 diabetic mice. C57BL/6J mice were fed a high-fat diet followed by low doses of streptozotocin to generate a
Islet amyloid develops diffusely throughout the pancreas before becoming severe and replacing endocrine cells.
Wang F, et al.
Diabetes, 50(11), 2514-2520 (2001)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service