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05-1108

Sigma-Aldrich

Anti-Insulin Antibody, clone1

clone MAB1, from mouse, purified by affinity chromatography

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Synonym(s):
Anti-IDDM, Anti-IDDM1, Anti-IDDM2, Anti-ILPR, Anti-IRDN, Anti-MODY10, Anti-PNDM4
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

MAB1, monoclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)

isotype

IgG1

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... INS(3630)

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This Item
05-1066I877305-386
antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

clone

MAB1, monoclonal

clone

E11D7, monoclonal

clone

polyclonal

clone

V284, monoclonal

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human, rat, mouse, rabbit, chicken

technique(s)

immunohistochemistry: suitable (paraffin)

technique(s)

ELISA: suitable, immunohistochemistry: suitable (paraffin)

technique(s)

neutralization: 3-12 μg/mL, western blot: 0.1-0.2 μg/mL

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG1

isotype

IgG1κ

isotype

-

isotype

IgG1

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General description

Insulin is a polypeptide hormone that regulates carbohydrate metabolism. Apart from being the primary agent in carbohydrate homeostasis, it has effects on fat metabolism.

Specificity

This antibody recognizes an epitope including the amino acid B30.

Immunogen

Biosynthetic human insulin

Application

Anti-Insulin Antibody, clone1 is an antibody against Insulin for use in IH(P).
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling

Quality

Evaluated by Immunohistochemistry in human pancreas tissue.
Immunohistochemistry Analysis: 1.25 µg/mL of this antibody detected insulin in human pancreas tissue.

Target description

Approx. 12 kDa

Linkage

Replaces: CBL71

Physical form

Affinity purified
The monoclonal is presented as 1mg in 1ml phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Storage and Stability

Store at 2-8°C and use within 1 year from date of receipt.

Analysis Note

Control
Human pancreas tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Gladys Teitelman
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 68(10), 691-702 (2020-10-02)
In pancreatic beta cells, proinsulin (ProIN) undergoes folding in endoplasmic reticulum/Golgi system and is translocated to secretory vesicles for processing into insulin and C-peptide by the proprotein convertases (PC)1/3 and PC2, and carboxypeptidase E. Human beta cells show significant variation
Renaud Jacquemart et al.
Computational and structural biotechnology journal, 14, 309-318 (2016-08-30)
The current processing paradigm of large manufacturing facilities dedicated to single product production is no longer an effective approach for best manufacturing practices. Increasing competition for new indications and the launch of biosimilars for the monoclonal antibody market have put

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