ZRB1138
Anti-Insulin Antibody, clone 3B5 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Ins
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
clone
recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 11.98 kDa
observed mol wt ~12 kDa
species reactivity
human, rat
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Clone 3B5 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects insulin.
Immunogen
KLH-conjugated linear peptide corresponding to 14 amino acids from the b-chain of human insulin.
Application
Quality Control Testing
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Insulin in Human Pancreas tissue lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected recombinant human Insulin.Affinity Binding Assay: A representative lot of this antibody bound Insulin peptide with a KD of 6.2 x 10-8 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:100 Dilution from a representative lot detected Insulin in human pancreas tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Insulin in RIN-mF5 cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Insulin in Human Pancreas tissue lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected recombinant human Insulin.Affinity Binding Assay: A representative lot of this antibody bound Insulin peptide with a KD of 6.2 x 10-8 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:100 Dilution from a representative lot detected Insulin in human pancreas tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Insulin in RIN-mF5 cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Insulin, clone 3B5 ZooMAb, Cat. No. ZRB1138, is a recombinant Rabbit monoclonal antibody that targets Insulin and has been tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
Insulin (UniProt: P01308) is encoded by the INS gene (Gene ID: 3630) in human. Insulin is an anabolic hormone produced by the b-cells of pancreas. Its major effects are to increase cell permeability of monosaccharides, amino acids, and fatty acids. It accelerates glycolysis and glycogen synthesis in the liver and reduces gluconeogenesis. It is also shown to increase oxidation of substrates in the Kreb s cycle. It counters the concerted action of a number of hyperglycemia generating hormones. Action of insulin on target cells is mediated via its interaction with insulin receptor (IR), a heterotetrameric glycoprotein consisting of two extracellular -subunits (135 kDa) and two transmembrane -subunits (95 kDa). IR functions as an allosteric enzyme in which the -subunit inhibits the tyrosine kinase activity of the -subunit. Insulin binding to the -subunits results in the stimulation of the tyrosine kinase activity of the -subunits, which results in phosphorylation of insulin-receptor substrates to propagate insulin signaling and activation of downstream signaling molecules, including PI 3-kinase. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mohan, C., et al. (1989). Curr. Topics Cell Regul. 30; 105-142; Posner, BI. (2017). Can. J. Diabetes. 41(1); 108-113).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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