1.05394

Granucult^® Enterobacteriaceae Enrichment Mossel broth

According to ISO 21528; For microbiology

• Synonym(s): EE Broth, MOSSEL Enterobacteriaceae enrichment broth, Enterobacteriaceae enrichment broth, MOSSEL Enterobacteriaceae enrichment broth, Enterobacteriaceae enrichment broth
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• agency: ISO 21528
• Quality Level: 200
• sterility: non-sterile
• form: granular
• manufacturer/tradename: GranuCult^® prime
• technique(s): microbiological culture: suitable
• pH: 7.3 (37 °C, 45 g/L in H₂O, after autoclaving)
• solubility: 45 g/L
• bulk density: 630 kg/m³
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: enterobacteriaceae

Description

General description

Granucult^® Enterobacteriaceae Enrichment Mossel broth is intended for the selective enrichment of Enterobacteriaceae. The undesired accompanying bacterial flora is inhibited by brilliant green and ox bile. Glucose favors the growth of all Enterobacteriaceae, and the high buffering capacity of the culture medium prevents the formed acid from killing the culture. Glucose is the main energy source and supports the rapid growth of all Enterobacteriaceae, while enzymatic digest of animal tissue provides nitrogen, vitamins, and amino acids.

Application

Granucult^® Enterobacteriaceae Enrichment Mossel broth is intended for the selective enrichment of Enterobacteriaceae from food and animal feed and other materials.

Analysis Note

Appearance (clearness): clear
Appearance (color): green
pH-value (25 °C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum E.coli resp. Salmonella and E.faecalis.
Additionaly monoculture of E.faecalis
Confirmation on VRBD agar resp. Tryptic soy agar

Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≤ 100
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≤ 100
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ≥ 1000
Growth on VRBD-Agar(Escherichia coli ATCC 8739 (WDCM 00012)): >10 colonies
Growth on VRBD-Agar(Escherichia coli ATCC 25922 (WDCM 00013)): >10 colonies
Growth on VRBD-Agar(Salmonella typhimurium ATCC 14028 (WDCM 00031)): >10 colonies
Growth on VRBD-Agar(Salmonella enteritidis ATCC 13076 (WDCM 00030)): >10 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total inhibition
Colonies (Escherichia coli ATCC 8739 (WDCM 00012)): pink to red colonies with or without precipitation halo
Colonies (Escherichia coli ATCC 25922 (WDCM 00013)): pink to red colonies with or without precipitation halo
Colonies (Salmonella typhimurium ATCC 14028 (WDCM 00031)): pink to red colonies with or without precipitation halo
Colonies (Salmonella enteritidis ATCC 13076 (WDCM 00030)): pink to red colonies with or without precipitation halo
Incubation:
EE broth: 24 ± 2 hours at 37 ± 1 °C aerobic
Confirmation on VRBD agar resp. Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C aerobic

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3