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2,3,5-Triphenyltetrazolium chloride

Color indicator. Supplement in culture media for the microbiological analysis of food and water samples

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Synonym(s):
2,3,5-Triphenyltetrazolium chloride, TTC, TT, TPTZ, TTZ, Tetrazolium Red
Empirical Formula (Hill Notation):
C19H15ClN4
CAS Number:
Molecular Weight:
334.80
Beilstein/REAXYS Number:
3923356
MDL number:

Quality Level

assay

≥98% (calculated on dried substance, perchloric acid titration)

form

solid

pH

3.7 (20 °C, 10 g/L in H2O)

mp

243 °C (decomposition)

solubility

150 g/L

bulk density

230 kg/m3

application(s)

microbiology

storage temp.

2-30°C

SMILES string

[Cl-].c1ccc(cc1)-c2nn(-c3ccccc3)[n+](n2)-c4ccccc4

InChI

1S/C19H15N4.ClH/c1-4-10-16(11-5-1)19-20-22(17-12-6-2-7-13-17)23(21-19)18-14-8-3-9-15-18;/h1-15H;1H/q+1;/p-1

InChI key

PKDBCJSWQUOKDO-UHFFFAOYSA-M

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1 of 4

This Item
T88771777988190
vibrant-m

T8877

2,3,5-Triphenyltetrazolium chloride

vibrant-m

88190

Tetrazolium Blue Chloride

pH

3.7 (20 °C, 10 g/L in H2O)

pH

-

pH

-

pH

-

solubility

150 g/L

solubility

H2O: 50 mg/mL

solubility

-

solubility

methanol: 0.5 g/10 mL ( hot)

application(s)

microbiology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

agriculture
environmental
food and beverages, microbiology

application(s)

microbiology

storage temp.

2-30°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

Quality Level

200

Quality Level

-

Quality Level

100

Quality Level

-

General description

2,3,5-triphenyltetrazolium chloride (TTC) is a dye largely used for enumeration of microbial colonies in solid culture media, being a key component of the dry rehydratable film system used for microbiological analysis of food. This dye is colorless in the oxidized form and red when reduced by microorganisms, due to formation of formazan. Live microorganisms reduce TTC by enzymatic action, forming red color formazan that is kept inside granules in the cells.

Application

2,3,5-triphenyltetrazolium chloride (TTC) is an indicator recommended for the microbiological analysis of food.

Analysis Note

Assay (perchloric acid titration, calculated on dried substance): ≥ 98 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λmax. (water): 245 - 248 nm
Spec. Absorptivity A 1%/1cm (λmax; 0,01 g/l; water; calculated on dried substance): ≥ 770
TLC-Test: passes test
Loss on drying (70 °C; ≤ 1 hPa): ≤ 0.5 %
Suitability for testing the germinability of seeds: passes test
Suitability for microbiology: passes test

pictograms

FlameExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Sol. 1 - Skin Irrit. 2

Storage Class

4.1B - Flammable solid hazardous materials

wgk_germany

WGK 3


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