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Millipore

MRS broth

(DE MAN, ROGOSA and SHARPE) GranuCult®, for the isolation, cultivation, and enrichment of Lactobacillus species

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Synonym(s):
Lactobacillus broth, Lactobacillus broth acc. to de MAN, ROGOSA and SHARPE, Lactobacillus broth

Quality Level

form

medium granules (dehydrated (DCM))

manufacturer/tradename

GranuCult®

technique(s)

microbiological culture: suitable

pH

5.5-5.9 (30 °C, 50 g/L in H2O, after autoclaving)

solubility

50 g/L

bulk density

620 kg/m3

application(s)

food and beverages
microbiology

storage temp.

2-8°C

suitability

Lactobacillus spp.

Related Categories

General description

The MRS broth facilitates luxuriant growth of Lactobacilli species from various sources, such as dairy products, faecal matter, and others. It contains polysorbate, acetate, magnesium, and manganese, which are known to act as special growth factors for Lactobacilli, as well as a rich nutrient base. Sodium acetate and ammonium citrate inhibits the growth of Streptococci, molds, and many other microorganisms.

Application

MRS broth media is specially designed for the isolation and cultivation of Lactobacillus species from a variety of sources, while inhibiting the growth of other competing microorganisms

Features and Benefits

  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
  • Excellent wettability, solubility, and free flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions
  • High batch-to- batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allowing many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear
Appearance (color): brown
pH-value (25 °C): 5.5 - 5.9
Growth promotion test in accordance with the current version of DIN EN ISO 11133.

Growth (Lactobacillus acidophilus ATCC 4356 (WDCM 00098)): good to very good
Growth (Lactobacillus sakei ATCC 15521 (WDCM00015)): good to very good
Growth (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016)): good to very good
Growth (Pediococcus pentosaceus ATCC 33316 (WDCM00158)): good to very good
Growth (Pediococcus damnosus ATCC 29358 (WDCM 00022)): good to very good
Incubation: up to 48 hours at 30 ± 1 °C, aerobic.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


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