38944

MRS Broth modified, Vegitone

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): DeMan, Rogosa and Sharpe Agar modified, Vegitone, Vegitone MRS Broth modified
• UNSPSC Code: 41171607
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: ammonium citrate, 2 g/L ; bromocresol green, 0.04 g/L ; captan, 0.004 g/L ; dextrose, 20 g/L ; dipotassium phosphate, 2 g/L ; magnesium sulfate, 0.10 g/L ; manganese sulfate, 0.05 g/L ; 2-phenylethyl alcohol, 3 g/L ; proteose peptone (vegetable), 10 g/L ; sodium acetate, 5 g/L ; yeast extract, 5 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 4.3±0.2 (25 °C)
• application(s): agriculture; bioburden testing; environmental; food and beverages; microbiology
• suitability: selective by low pH for Bifidobacterium spp.; selective by low pH for Lactobacillus spp.

Description

Application

This MRS Broth contains plant peptone instead of animal peptone. It is recommended for the isolation and cultivation of Lactobacillus species.

Preparation Note

Suspend 47.2 g in 1000 ml distilled water containing 1 ml polysorbate 80 (TWEEN^® 80; Sigma P8074). Boil to dissolve the medium completely and mix thoroughly. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If necessary adjust the pH with glacial acetic acid after sterilization.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type N95 (US)