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MRS Broth modified, Vegitone

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
DeMan, Rogosa and Sharpe Agar modified, Vegitone, Vegitone MRS Broth modified
UNSPSC Code:
41171607
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

ammonium citrate, 2 g/L
bromocresol green, 0.04 g/L
captan, 0.004 g/L
dextrose, 20 g/L
dipotassium phosphate, 2 g/L
magnesium sulfate, 0.10 g/L
manganese sulfate, 0.05 g/L
2-phenylethyl alcohol, 3 g/L
proteose peptone (vegetable), 10 g/L
sodium acetate, 5 g/L
yeast extract, 5 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

4.3±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages

microbiology

suitability

selective by low pH for Bifidobacterium spp.
selective by low pH for Lactobacillus spp.

Related Categories

Application

This MRS Broth contains plant peptone instead of animal peptone. It is recommended for the isolation and cultivation of Lactobacillus species.

Preparation Note

Suspend 47.2 g in 1000 ml distilled water containing 1 ml polysorbate 80 (TWEEN® 80; Sigma P8074). Boil to dissolve the medium completely and mix thoroughly. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If necessary adjust the pH with glacial acetic acid after sterilization.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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