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Millipore

Bactident E. coli

suitable for detection

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Synonym(s):
Test on E. Coli

feature

ready-to-use

Quality Level

manufacturer/tradename

Bactident

technique(s)

microbe id | specific enzyme detection: suitable

input

sample(s) (environmental)
water

application(s)

detection
environmental
food and beverages

storage temp.

2-8°C

suitability

Citrobacter spp.
Escherichia coli
Proteus spp.

General description

BACTIDENT E. coli test strips are impregnated with 4-methylumbelliferyl-β-D-glucuronide (MUG), which is cleaved to form 4-methylumbelliferone in the presence of β-D-Glucuronidase. The light blue fluorescence upon excitation with long-wavelength UV light, indicates the presence of the enzyme. Tryptophanase activity is confirmed if the bacterial suspension turns red upon the addition of KOVACS’ reagent due to indole formation.
rapid identification kit for E. coli

Application

BACTIDENT E. coli is used for the bioburden testing in drinking water. This test allows for the rapid detection of coliform organisms (E. coli) by testing the bacterial culture for the presence of enzymes β-D-Glucuronidase and Tryptophanase.

Linkage

Replaces: 1133030001

Analysis Note

Fluorescence
- Escherichia coli ATCC 25922 (WDCM 00013):
+
- Escherichia coli 194: +
- Escherichia coli ATCC 4351: +
- Proteus hauseri ATCC 13315: -
- Citrobacter freundii ATCC 8090: -
Indole formation
- Escherichia coli ATCC 25922 (WDCM 00013):
+
- Escherichia coli 194: +
- Escherichia coli ATCC 4351: +
- Proteus hauseri ATCC 13315: +
- Citrobacter freundii ATCC 8090: -

Legal Information

BACTIDENT is a trademark of Merck KGaA, Darmstadt, Germany

signalword

Danger-Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1 - Skin Sens. 1

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

96.8 °F

flash_point_c

36 °C


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