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1.15939

Sigma-Aldrich

Nuclear fast red (C.I. 60760)

for microscopy Certistain®

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Synonym(s):
Nuclear fast red (C.I. 60760)
Empirical Formula (Hill Notation):
C14H8NNaO7S
CAS Number:
Molecular Weight:
357.27
MDL number:
EC Index Number:
229-088-0

Quality Level

form

solid

composition

Dye, ≥90% spectrophotometric assay

pH

5.5-7.0 (20 °C, 1 g/L in H2O)

solubility

1 g/L

bulk density

400‑450 kg/m3

εmax

380-422 at 557-559 nm in DMSO at 0.02 g/L
383-426 at 520-522 nm in DMSO at 0.02 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C14H9NO7S.Na/c15-9-7-8(12(18)14(13(9)19)23(20,21)22)11(17)6-4-2-1-3-5(6)10(7)16;/h1-4,18-19H,15H2,(H,20,21,22);/q;+1/p-1

InChI key

IFSXZLJQEKGQAF-UHFFFAOYSA-M

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1 of 4

This Item
N30201.0417160700
vibrant-m

1.15939

Nuclear fast red (C.I. 60760)

vibrant-m

N3020

Nuclear Fast Red Solution

vibrant-m

1.04171

Nuclear fast red (C.I. 60760)

vibrant-m

60700

Nuclear Fast Red

εmax

380-422 at 557-559 nm in DMSO at 0.02 g/L, 383-426 at 520-522 nm in DMSO at 0.02 g/L

εmax

-

εmax

-

εmax

≥275 at 550-560 nm in DMSO, ≥300 at 516-526 nm in DMSO

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

form

solid

form

solution

form

solid

form

powder

solubility

1 g/L

solubility

-

solubility

1 g/L

solubility

methanol: water (1:1): 0.01 g/10 mL, red

composition

Dye, ≥90% spectrophotometric assay

composition

-

composition

Dye, ≥90% spectrophotometric assay

composition

-

General description

Nuclear fast red (C.I. 60760) for microscopy Certistain®, is a dry dye for microscopy that is often used for staining nuclei in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of a Nuclear fast red staining solution. It is commonly used for counterstaining in histochemical reactions, e.g. in Berlin blue staining for detection of iron or in silver plating of reticulin fibers, as well as nuclear staining with Alcian blue. As the dye belongs to the portfolio of Certistain® dyes, it is chemically analyzed according to strict specifications before release (CE marked and IVD product) and a high staining quality, with reliable staining results is guaranteed. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 90 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ 1 (Dimethyl sulfoxide): 557 - 559 nm
Absorption maximum λ 2 (Dimethyl sulfoxide): 520 - 522 nm
Spec. Absorptivity A 1%/1cm
- λ1max; 0.02 g/l; dimethyl sulphoxide:
380 - 422
- λ2max; 0.02 g/l; Dimethyl sulphoxide: 383 - 426
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_c

Not applicable


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