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60700

Sigma-Aldrich

Nuclear Fast Red

for microscopy (Bot., Hist.)

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Synonym(s):
4-Amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt
Empirical Formula (Hill Notation):
C14H8NNaO7S
CAS Number:
Molecular Weight:
357.27
Colour Index Number:
60760
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

for microscopy (Bot., Hist.)

Quality Level

form

powder

solubility

methanol: water (1:1): 0.01 g/10 mL, red

εmax

≥275 at 550-560 nm in DMSO
≥300 at 516-526 nm in DMSO

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].Nc1c(O)c(c(O)c2C(=O)c3ccccc3C(=O)c12)S([O-])(=O)=O

InChI

1S/C14H9NO7S.Na/c15-9-7-8(12(18)14(13(9)19)23(20,21)22)11(17)6-4-2-1-3-5(6)10(7)16;/h1-4,18-19H,15H2,(H,20,21,22);/q;+1/p-1

InChI key

IFSXZLJQEKGQAF-UHFFFAOYSA-M

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This Item
N8002F2256N3020
Nuclear Fast Red for microscopy (Bot., Hist.)

Sigma-Aldrich

60700

Nuclear Fast Red

Nuclear Fast Red

Sigma-Aldrich

N8002

Nuclear Fast Red

Fast Red RC Salt

Sigma-Aldrich

F2256

Fast Red RC Salt

form

powder

form

powder

form

powder

form

solution

solubility

methanol: water (1:1): 0.01 g/10 mL, red

solubility

water: soluble 4 mg/mL, dark red to very dark red and red purple

solubility

water: 1 mg/mL

solubility

-

εmax

≥275 at 550-560 nm in DMSO, ≥300 at 516-526 nm in DMSO

εmax

-

εmax

-

εmax

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

General description

Nuclear Fast Red (C.l. 60760) is a substituted hydroxy anthraquinone chromophore that can chelate metal ions. It is also known as kernechtrot and 4-Amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt. It is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. It is a dry staining dye that makes target structures (by fixing, wherenecessary embedding, staining with the above nuclear fast red solution, counterstaining, mounting) in human-histological specimen materials, for examplehistological sections of e. g. the kidney, evaluable for diagnostic purposes.

Application

Nuclear fast red has been used for the nuclear staining of PB (Prussian blue) positive cells in the rostral sub-epidermis and upper beak coronal region isolated from pigeons.Recent applications include:
  • staining sperm and spermatids in semen
  • identifying areas for PCR analysis in paraffin-embedded tissues
  • nuclear counterstaining following immunostaining and silver staining
Used as a biological stain

Biochem/physiol Actions

Nuclear fast red dye has anthraquinonic nature and is used either alone or in combination with other dyes. It is mainly used for staining of nuclei.

Principle

Nuclear fast red can be used for simple nuclear staining. It is an acidic dyebelonging to the group of anthraquinone dyes. Aluminum sulfate, functioning as a mordant, is added to form a color lake that enables red nuclear staining. Nuclear fast red-aluminum sulfate is used for counterstaining in histochemicalreactions, e. g. in Berlin blue staining for detection of iron or in silver staining. Nuclear fast red for microscopy Certistain® can also be used fornuclear staining with alcian blue.

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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