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126579

Sigma-Aldrich

Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free

Synonym(s):

Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free

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About This Item

CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.25

assay

≥95% (Albumin, electrophoresis)

form

lyophilized powder

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤0.2% fatty acids
≤2.0% Ash
≤5% moisture
≤50 EU/mg endotoxins

color

pale green

solubility

aqueous buffer: 20 mg/mL
water: 20 mg/mL

shipped in

ambient

storage temp.

2-8°C

General description

Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free is designed for use in cell culture systems and animal perfusion studies. It has a pH of 7.0 (1% solution).
Bovine serum albumin (BSA), a member of the serum albumin family, contains three domains and two sub-domains under each. It is a non-glycosylated globular, α-helical protein that has 17-disulfide bonds.

Application

Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free has been used in the preparation of advanced glycation end-products (AGEs). It has also been used to dilute lipopolysaccharide (LPS) and tumor necrosis factor (TNF) and in the stimulation and fixation of whole blood cells.

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is the most important constituent of cell culture media. It aids embryonic stem cells (hESC) differentiation. BSA facilitates the transport of drugs, hormones, and fatty acids. It elicits high solubility and is suitable in the cosmetic and pharmaceutical industries. It is applicable as a blocking agent in enzyme-linked immunosorbent assay (ELISA).

Warning

Toxicity: Standard Handling (A)

Preparation Note

Material may take up to 24 h to fully dissolve.

Reconstitution

Following reconstitution, store in the refrigerator (4°C). 0.1% NaN₃ may be added as a preservative. Stock solutions are stable for up to 3 months at 4°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Dan Ye et al.
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Novel strategies are needed to modulate β-cell differentiation and function as potential β-cell replacement or restorative therapies for diabetes. We previously demonstrated that small molecules based on the isoxazole scaffold drive neuroendocrine phenotypes. The nature of the effects of isoxazole

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