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1.52006

Supelco

Chromolith® RP-18 endcapped

L × I.D. 100 mm x 2 mm HPLC column

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Synonym(s):
Chromolith® Performance RP-18 endcapped 100-2 HPLC column

material

PEEK column

Quality Level

agency

suitable for USP L1

product line

Chromolith®

feature

endcapped

packaging

1 ea of

extent of labeling

18% Carbon loading

parameter

200 bar pressure
45 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

10 cm × 2 mm

matrix

monolithic silica

matrix active group

C18 bonding phase

pore size

1.5 μm macroporosity
13 nm mesoporosity

operating pH range

2-7.5

separation technique

reversed phase

storage temp.

no temp limit

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This Item
1.520011.021291.51450
Chromolith® RP-18 endcapped L × I.D. 100 mm x 2 mm HPLC column

1.52006

Chromolith® RP-18 endcapped

Chromolith® RP-18 endcapped L × I.D. 100 mm x 3 mm HPLC column

1.52001

Chromolith® RP-18 endcapped

Chromolith® RP-18 endcapped L × I.D. 100 mm x 4.6 mm HPLC column

1.02129

Chromolith® RP-18 endcapped

Chromolith® RP-18 endcapped L × I.D.50 mm x 4.6 mm HPLC column

1.51450

Chromolith® RP-18 endcapped

matrix active group

C18 bonding phase

matrix active group

C18 bonding phase

matrix active group

C18 bonding phase

matrix active group

C18 bonding phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

product line

Chromolith®

product line

Chromolith®

product line

Chromolith®

product line

Chromolith®

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

technique(s)

HPLC: suitable, UHPLC-MS: suitable, mass spectrometry (MS): suitable (High), LC/MS: suitable, UHPLC: suitable

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

General description

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® Performance RP-18 endcapped 100-2 columns have macropores with 1.5 μm diameter, giving a column efficiency exceeding 100,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilane with full end-capping. Chromolith® Performance RP-18 endcapped 100-2 columns fall under the USP L1 Classification. The column efficiency is comparable to a 3-4 μm particle size fully porous particulate column. Chromolith® Performance RP-18 endcapped 100-2 columns are perfectly suitable for LC-MS applications.
The column dimension of 100 mm length and 2 mm I.D. is the best choice for fast separations of complex samples on any U/HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith® RP-18 endcapped 5-2 guard cartridges (3 pieces) [1.52009] with the Chromolith® Guard Cartridge Holder (kit) 5 mm x 2 mm [1.52004]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Analysis Note

Appearance: passes test
Pressure: ≤ 55 bar
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 100000
Capacity factor (Anthracene): 3.9 - 4.6
Tailing (USP): 0.90 - 1.30 %
Selectivity
separation factor (anthracene/progesterone): 2.30 - 2.60

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articles

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

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