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Chromolith® HighResolution RP-18 endcapped

L × I.D. 25 mm x 4.6 mm HPLC column

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Synonym(s):
Chromolith® HighResolution RP-18 endcapped 25-4.6 HPLC column

material

PEEK column

Quality Level

agency

suitable for ISO/CEN 6304.09
suitable for USP L1

product line

Chromolith®

feature

endcapped
endcapped

packaging

1 ea of

extent of labeling

15% Carbon loading
18% Carbon loading

parameter

200 bar pressure
200 bar pressure
45 °C max. temp.
50 °C max. temp.

technique(s)

HPLC: suitable
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
mass spectrometry (MS): suitable (High)

column L × I.D.

2.5 cm × 4.6 mm

surface area

250 m2/g × 250 m2/g

matrix

highly porous
monolithic silica
monolithic silica

matrix active group

C18 bonding phase, octadecylsilane (ODS)
C18 bonding phase

pore size

1.15 μm macroporosity
1.5 μm macroporosity
15 nm mesoporosity
15 nm mesoporosity

operating pH range

2-7.5
2-7.5

separation technique

reversed phase

storage temp.

no temp limit

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This Item
1.520221.520231.52021
matrix active group

C18 bonding phase, octadecylsilane (ODS)

matrix active group

C18 bonding phase, octadecylsilane (ODS)

matrix active group

C18 bonding phase, octadecylsilane (ODS)

matrix active group

C18 bonding phase, octadecylsilane (ODS)

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

agency

suitable for ISO/CEN 6304.09, suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

pore size

1.15 μm macroporosity, 15 nm mesoporosity, 1.5 μm macroporosity

pore size

1.15 μm macroporosity, 1.5 μm macroporosity, 15 nm mesoporosity

pore size

1.15 μm macroporosity, 1.5 μm macroporosity, 15 nm mesoporosity

pore size

1.15 μm macroporosity, 1.5 μm macroporosity, 15 nm mesoporosity

General description

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® HighResolution RP-18 endcapped columns have macropores with 1.15 μm diameter, giving a column efficiency exceeding 140,000 plates/meter. The mesopores are 15 nm (150 Å), the surface modification is octadecylsilane with full end-capping.

Chromolith® HighResolution RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparableto a 2.7 to 3 μm particle size fully porous particulate column. Chromolith® HighResolution RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 25 mm length and 4.6 mm I.D. is the best choice for very rapid separations on any HPLC System and rapid method development.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith® HighResolution RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.52025] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Chromolith® HighResolution RP-18 endcapped columns are also available in 2 mm I.D. column dimension for perfect fit to UHPLC and UHPLC-MS needs.
Flyer: UHPLC-like performance - Rapid and Robust at Low Pressure - Chromolith® HighResolution RP-18 endcapped 2 mm I.D. HPLC Columns

Analysis Note

Pressure: 10 - 30 bar
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 110000
Capacity factor (Anthracene): 3.3 - 4.6
Tailing (USP): 0.90 - 1.40 %
Selectivity
separation factor (anthracene/progesterone): 1.85 - 2.35

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articles

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

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