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1.52370

Supelco

Chromolith® WP 300 RP-18

L × I.D. 10 cm × 2 mm HPLC column

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Synonym(s):
Chromolith® WP 300 RP-18 100-2 HPLC column

material

PEEK column

Quality Level

agency

suitable for USP L1

product line

Chromolith®

feature

endcapped: no

packaging

1 ea of

extent of labeling

18% loading

parameter

200 bar pressure
60 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable

column L × I.D.

10 cm × 2 mm

surface area

300 m2/g

matrix

monolithic silica

matrix active group

C18 bonding phase

pore size

2 μm macroporosity
30 mm mesoporosity

operating pH range

2-7.5

separation technique

reversed phase

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1 of 4

This Item
1.522721.522681.52267
Chromolith® WP 300 RP-18 L × I.D. 10 cm × 2 mm HPLC column

1.52370

Chromolith® WP 300 RP-18

Chromolith® WP 300 RP-18 L × I.D. 10 mm x 4.6 mm guard cartridges (3 pieces)

1.52272

Chromolith® WP 300 RP-18

Chromolith® WP 300 RP-8 L × I.D. 5 mm x 4.6 mm guard cartridges (3 pieces)

1.52268

Chromolith® WP 300 RP-8

Chromolith® WP 300 RP-8 L × I.D. 10 mm x 4.6 mm guard cartridges (3 pieces)

1.52267

Chromolith® WP 300 RP-8

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L7

agency

suitable for USP L7

product line

Chromolith®

product line

Chromolith®

product line

Chromolith®

product line

Chromolith®

feature

endcapped: no

feature

endcapped: no

feature

endcapped: no

feature

endcapped: no

packaging

1 ea of

packaging

pkg of 3 ea

packaging

pkg of 3 ea

packaging

pkg of 3 ea

extent of labeling

18% loading

extent of labeling

18% Carbon loading, 18% loading

extent of labeling

4-5% Carbon loading

extent of labeling

4-5% Carbon loading

General description

The most hydrophobic of the Chromolith® WP 300 line, the RP-18 column is useful for the resolution of peptides and smaller proteins. One critical quality attribute (CQA) required by regulatory bodies is the peptide map of a biotherapeutic. Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RPHPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information.
The Chromolith® WP 300 RP-18 column gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry incorporated into the Chromolith® WP 300 line improve resolution by increasing efficiency and reducing peak tailing.

Chromolith® WP 300 columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® WP 300 RP-18, 2 mm I.D. columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 30 nm (300 Å), the surface modification is Octadecylsilane. Chromolith® WP 300 RP-18 columns fall under the USP L1 Classification. Chromolith® WP 300 RP-18 columns are fully suitable for LC-MS applications.
The column dimension of 100 mm length and 2 mm I.D. is the best choice for fast separations of Biomolecules on any UHPLC System.

Chromolith® WP 300 RP-18, 100-2 HPLC columns enable:
—Completely bioinert column hardware and high biorecovery
—High-speed separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith® WP 300 RP-18, 5-2 guard cartridges (3 pieces) [1.52372] with the Chromolith® Guard Cartridge Holder Bioinert 5 mm x 2 mm [1.52355]

The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series. [1.51467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articles

Comparative analysis of different columns in resolving medium-sized fragments of monoclonal antibodies, after digestion using dithiothreitol (DTT) or IdeS (a protease), by Reversed-Phase Chromatography.

Related Content

Analytical Chromolith® HPLC columns for Bio applications, 300A mesopores: RP-18, RP-8, RP-4, Prot-A, Epoxy

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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