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20-133-M

Sigma-Aldrich

PKC Lipid Activator

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eCl@ss:
32160405
NACRES:
NA.41

form

liquid

Quality Level

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

UniProt accession no.

Gene Information

human ... PRKCA(5578)

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R1031SAB5701912SAB4502380
Sigma-Aldrich

20-133-M

PKC Lipid Activator

Sigma-Aldrich

R1031

RSK1, active, GST tagged human

form

liquid

form

buffered aqueous glycerol solution

form

liquid

form

buffered aqueous solution

Gene Information

human ... PRKCA(5578)

Gene Information

human ... RPS6KA1(6195)

Gene Information

human ... PRKCZ(5590)

Gene Information

human ... PRKCZ(5590)

UniProt accession no.

P17252

UniProt accession no.

Q15418

UniProt accession no.

Q05513

UniProt accession no.

Q05513

NCBI accession no.

NM_002737.2

NCBI accession no.

-

NCBI accession no.

-

NCBI accession no.

Q05513

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

General description

Protein kinase C (PKC) belongs to a family of serine/ threonine kinases. It comprises a regulatory domain at the N-terminus and a catalytic domain at the C-terminus. The regions are divided into conserved regions (C1–C4) and regions that differ between isoforms (V1–V5). PKC has a common pseudosubstrate domain in the regulatory region.

Application

For use in kinase assays.
Research Category
All

Biochem/physiol Actions

Protein kinase C (PKC) is activated by diacylglycerol (DAG) and acts as an intracellular receptor of tumor-promoting phorbol esters. It regulates several cell functions and signal transduction pathways through cell migration and polarity, differentiation, proliferation, and cell death. PKC is part of a large group of phospholipid-dependent enzymes, which catalyze the covalent transfer of phosphate from ATP to serine and threonine residues of proteins.

Physical form

20mM MOPS, pH 7.2, 25mM β-glycerolphosphate, 1mM sodium orthovanadate, 1mM dithiothreitol, 1mM CaCl2.

Storage and Stability

1 year at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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D Breitkreutz et al.
Journal of cancer research and clinical oncology, 133(11), 793-808 (2007-07-31)
The protein kinase C (PKC) family represents a large group of phospholipid dependent enzymes catalyzing the covalent transfer of phosphate from ATP to serine and threonine residues of proteins. Phosphorylation of the substrate proteins induces a conformational change resulting in

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